Neutrophil-associated activation markers in healthy smokers relates to a fall in DLCO and to emphysematous changes on high resolution CT

A. Ekberg-Jansson, B. Andersson, B. Bake, M. Boijsen, I. Enanden, A. Rosengren, B. E. Skoogh, Ulf Tylen, P. Venge, C. G. Löfdahl

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Smoking is a risk factor for developing chronic obstructive pulmonary disease (COPD), but there are no good indicators for early identification of subjects who will develop symptomatic COPD. The aim of this study was to investigate inflammatory mechanisms related to changes in lung function and emphysematous changes on high resolution computed tomography (HRCT) in 'healthy' smokers. Subjects were 60-year-old men from a population study. Bronchoscopy was performed in 30 smokers and 18 who had never smoked. Blood tests, lung function measurements and HRCT were carried out in 58 and 34 subjects, respectively. In comparison with never-smokers, smokers had higher levels of myeloperoxidase (MPO), human neutrophil lipocalin (HNL), eosinophil cationic protein (ECP) and lysozyme in blood, higher levels of MPO, interleukin-8 (IL-8) and HNL in bronchial lavage (BL), and of IL-8, HNL and interleukin-1β (IL-1β) in bronchoalveolar lavage (BAL). Smokers also had lower levels of Clara cell protein 16 (CC-16) in blood. HNL in BL and BAL showed strong correlations to other inflammatory markers (MPO, IL-8, IL-1β). The variations in MPO in BL were explained by variations in HNL (R2=0.69), while these variations in BAL were explained by variations in HNL and IL-1β (R2=0.76). DLCO was the lung function variable most closely related to MPO and IL-8 in BL and BAL and to IL-1β in BAL. In a multiple regression analysis, MPO, IL-1β, IL-8 and CC-16 in BL and MPO in BAL contributed to the explanation of variations in DLCO to 41% and 22%, respectively, independent of smoking habits. In smokers with emphysematous lesions on HRCT, HNL in BAL correlated to emphysema score (rs=0.71). We conclude that 'healthy' smoking men with a near normal FEV1 show signs of inflammation in the lower airways that are related to a decrease in DLCO and to emphysematous lesions on HRCT. This inflammation seems to be the result of both monocyte/macrophage and neutrophil activation.

Original languageEnglish (US)
Pages (from-to)363-373
Number of pages11
JournalRespiratory Medicine
Volume95
Issue number5
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Neutrophil Activation
Bronchoalveolar Lavage
Lipocalins
Peroxidase
Neutrophils
Interleukin-8
Interleukin-1
Lipocalin 1
Tomography
Smoking
Lung
Chronic Obstructive Pulmonary Disease
Eosinophil Cationic Protein
Inflammation
Macrophage Activation
Emphysema
Hematologic Tests
Bronchoscopy
Muramidase
Habits

Keywords

  • Bronchoalveolar lavage
  • Chronic obstructive pulmonary disease
  • Computed tomography
  • Cytokines
  • Inflammation
  • Lung function
  • Smoking

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Ekberg-Jansson, A., Andersson, B., Bake, B., Boijsen, M., Enanden, I., Rosengren, A., ... Löfdahl, C. G. (2001). Neutrophil-associated activation markers in healthy smokers relates to a fall in DLCO and to emphysematous changes on high resolution CT. Respiratory Medicine, 95(5), 363-373. https://doi.org/10.1053/rmed.2001.1050

Neutrophil-associated activation markers in healthy smokers relates to a fall in DLCO and to emphysematous changes on high resolution CT. / Ekberg-Jansson, A.; Andersson, B.; Bake, B.; Boijsen, M.; Enanden, I.; Rosengren, A.; Skoogh, B. E.; Tylen, Ulf; Venge, P.; Löfdahl, C. G.

In: Respiratory Medicine, Vol. 95, No. 5, 2001, p. 363-373.

Research output: Contribution to journalArticle

Ekberg-Jansson, A, Andersson, B, Bake, B, Boijsen, M, Enanden, I, Rosengren, A, Skoogh, BE, Tylen, U, Venge, P & Löfdahl, CG 2001, 'Neutrophil-associated activation markers in healthy smokers relates to a fall in DLCO and to emphysematous changes on high resolution CT', Respiratory Medicine, vol. 95, no. 5, pp. 363-373. https://doi.org/10.1053/rmed.2001.1050
Ekberg-Jansson, A. ; Andersson, B. ; Bake, B. ; Boijsen, M. ; Enanden, I. ; Rosengren, A. ; Skoogh, B. E. ; Tylen, Ulf ; Venge, P. ; Löfdahl, C. G. / Neutrophil-associated activation markers in healthy smokers relates to a fall in DLCO and to emphysematous changes on high resolution CT. In: Respiratory Medicine. 2001 ; Vol. 95, No. 5. pp. 363-373.
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abstract = "Smoking is a risk factor for developing chronic obstructive pulmonary disease (COPD), but there are no good indicators for early identification of subjects who will develop symptomatic COPD. The aim of this study was to investigate inflammatory mechanisms related to changes in lung function and emphysematous changes on high resolution computed tomography (HRCT) in 'healthy' smokers. Subjects were 60-year-old men from a population study. Bronchoscopy was performed in 30 smokers and 18 who had never smoked. Blood tests, lung function measurements and HRCT were carried out in 58 and 34 subjects, respectively. In comparison with never-smokers, smokers had higher levels of myeloperoxidase (MPO), human neutrophil lipocalin (HNL), eosinophil cationic protein (ECP) and lysozyme in blood, higher levels of MPO, interleukin-8 (IL-8) and HNL in bronchial lavage (BL), and of IL-8, HNL and interleukin-1β (IL-1β) in bronchoalveolar lavage (BAL). Smokers also had lower levels of Clara cell protein 16 (CC-16) in blood. HNL in BL and BAL showed strong correlations to other inflammatory markers (MPO, IL-8, IL-1β). The variations in MPO in BL were explained by variations in HNL (R2=0.69), while these variations in BAL were explained by variations in HNL and IL-1β (R2=0.76). DLCO was the lung function variable most closely related to MPO and IL-8 in BL and BAL and to IL-1β in BAL. In a multiple regression analysis, MPO, IL-1β, IL-8 and CC-16 in BL and MPO in BAL contributed to the explanation of variations in DLCO to 41{\%} and 22{\%}, respectively, independent of smoking habits. In smokers with emphysematous lesions on HRCT, HNL in BAL correlated to emphysema score (rs=0.71). We conclude that 'healthy' smoking men with a near normal FEV1 show signs of inflammation in the lower airways that are related to a decrease in DLCO and to emphysematous lesions on HRCT. This inflammation seems to be the result of both monocyte/macrophage and neutrophil activation.",
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AU - Andersson, B.

AU - Bake, B.

AU - Boijsen, M.

AU - Enanden, I.

AU - Rosengren, A.

AU - Skoogh, B. E.

AU - Tylen, Ulf

AU - Venge, P.

AU - Löfdahl, C. G.

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N2 - Smoking is a risk factor for developing chronic obstructive pulmonary disease (COPD), but there are no good indicators for early identification of subjects who will develop symptomatic COPD. The aim of this study was to investigate inflammatory mechanisms related to changes in lung function and emphysematous changes on high resolution computed tomography (HRCT) in 'healthy' smokers. Subjects were 60-year-old men from a population study. Bronchoscopy was performed in 30 smokers and 18 who had never smoked. Blood tests, lung function measurements and HRCT were carried out in 58 and 34 subjects, respectively. In comparison with never-smokers, smokers had higher levels of myeloperoxidase (MPO), human neutrophil lipocalin (HNL), eosinophil cationic protein (ECP) and lysozyme in blood, higher levels of MPO, interleukin-8 (IL-8) and HNL in bronchial lavage (BL), and of IL-8, HNL and interleukin-1β (IL-1β) in bronchoalveolar lavage (BAL). Smokers also had lower levels of Clara cell protein 16 (CC-16) in blood. HNL in BL and BAL showed strong correlations to other inflammatory markers (MPO, IL-8, IL-1β). The variations in MPO in BL were explained by variations in HNL (R2=0.69), while these variations in BAL were explained by variations in HNL and IL-1β (R2=0.76). DLCO was the lung function variable most closely related to MPO and IL-8 in BL and BAL and to IL-1β in BAL. In a multiple regression analysis, MPO, IL-1β, IL-8 and CC-16 in BL and MPO in BAL contributed to the explanation of variations in DLCO to 41% and 22%, respectively, independent of smoking habits. In smokers with emphysematous lesions on HRCT, HNL in BAL correlated to emphysema score (rs=0.71). We conclude that 'healthy' smoking men with a near normal FEV1 show signs of inflammation in the lower airways that are related to a decrease in DLCO and to emphysematous lesions on HRCT. This inflammation seems to be the result of both monocyte/macrophage and neutrophil activation.

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KW - Chronic obstructive pulmonary disease

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KW - Cytokines

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