Neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus

L. W S Chang, Y. C. Zee, R. F. Pritchett, Alex Ardans

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Infectious bovine rhinotracheitis virus (IBRV) has been shown in this report to have thirty-three polypeptides. Ten of the eleven polypeptides which can be labeled with (3H)-glucosamine are located on the surface of the virus since they can be surface labeled with sodium boro(3H)hydride. In order to define the immunologically important viral proteins, monoclonal antibodies were prepared against the virus and selected for their ability to neutralize infectivity. Four such hybridoma lines were obtained for characterization of the antigens that elicit neutralizing antibodies. The viral polypeptides were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and the specificity of each monoclonal antibody was determined by "Western" blot analysis and/or by immunoprecipitation of (35S)-methionine and (3H)-glucosamine labeled infected cell lysates by the monoclonal antibodies. One monoclonal antibody reacted with two glycoproteins, gp135 and gp78a, on the "Western" blot but immunoprecipitated three glycoproteins, gp135, gp78a, and gp54 from labeled infected cell lysates. The other three monoclonal antibodies immunoprecipitated a single glycoprotein, gp78b, from (3H)-glucosamine labeled infected cell lysates but not from (35S)-methionine labeled infected cell lysates.

Original languageEnglish (US)
Pages (from-to)203-215
Number of pages13
JournalArchives of Virology
Volume88
Issue number3-4
DOIs
StatePublished - Sep 1 1986

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Bovine Herpesvirus 1
Neutralizing Antibodies
Monoclonal Antibodies
Contactin 1
Glucosamine
Methionine
Peptides
Western Blotting
Viruses
Hybridomas
Viral Proteins
Immunoprecipitation
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Glycoproteins
Sodium
Antigens

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus. / Chang, L. W S; Zee, Y. C.; Pritchett, R. F.; Ardans, Alex.

In: Archives of Virology, Vol. 88, No. 3-4, 01.09.1986, p. 203-215.

Research output: Contribution to journalArticle

Chang, L. W S ; Zee, Y. C. ; Pritchett, R. F. ; Ardans, Alex. / Neutralizing monoclonal antibodies directed to infectious bovine rhinotracheitis virus. In: Archives of Virology. 1986 ; Vol. 88, No. 3-4. pp. 203-215.
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