Neutralizing antibody responses in Africa green monkeys naturally infected with simian immunodeficiency virus (SIVagm)

Michael M. Gicheru, Moses Otsyula, Paul Spearman, Barney S. Graham, Chris J Miller, Harriet L. Robinson, Nancy L. Haigwood, David C. Montefiori

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

This study assessed the magnitude and cross-reactivity of the neutralizing antibody response generated by natural SIV infection in wild-caught African green monkeys. Neutralizing antibodies of variable potency, sometimes exceeding a titer of 1:1,000, were detected in 20 of 20 SIV-seropositive African green monkeys in Kenya. Detection of those neutralizing antibodies was dependent on the strain of virus and the cells used for assay, where the most sensitive detection was made with SIVagm1532 in Sup T1 cells. Potent neutralization of SIVagm1532 was seen with contemporaneous autologous serum. Potent neutralization was also detected with laboratory-passaged SIVmac251 and SIVsmB670, but not with SIVsmE660 and two additional strains of SIVagm. Serum samples from rhesus macaques (Macaco mulatta) experimentally infected with either SIVmac251 or SIVsmE660 were capable of low-level neutralization of SIVagm. These results indicate that natural infection with SIV can generate strain-specific neutralizing antibodies in African green monkeys. They also indicate that some neutralization determinants of SIVagm are partially shared with SIV strains that arose in sooty mangabys and were subsequently transmitted to rhesus macaques.

Original languageEnglish (US)
Pages (from-to)97-104
Number of pages8
JournalJournal of Medical Primatology
Volume28
Issue number3
StatePublished - Jun 1999

Fingerprint

Simian immunodeficiency virus
Cercopithecus aethiops
Simian Immunodeficiency Virus
Neutralizing Antibodies
neutralizing antibodies
neutralization
Antibody Formation
monkeys
Macaca mulatta
Kenya
Infection
Serum
cross reaction
infection
cells
Viruses
viruses
assays
sampling

Keywords

  • Antibodies
  • Neutralization
  • Nonhuman
  • Primates
  • SIV

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Gicheru, M. M., Otsyula, M., Spearman, P., Graham, B. S., Miller, C. J., Robinson, H. L., ... Montefiori, D. C. (1999). Neutralizing antibody responses in Africa green monkeys naturally infected with simian immunodeficiency virus (SIVagm). Journal of Medical Primatology, 28(3), 97-104.

Neutralizing antibody responses in Africa green monkeys naturally infected with simian immunodeficiency virus (SIVagm). / Gicheru, Michael M.; Otsyula, Moses; Spearman, Paul; Graham, Barney S.; Miller, Chris J; Robinson, Harriet L.; Haigwood, Nancy L.; Montefiori, David C.

In: Journal of Medical Primatology, Vol. 28, No. 3, 06.1999, p. 97-104.

Research output: Contribution to journalArticle

Gicheru, MM, Otsyula, M, Spearman, P, Graham, BS, Miller, CJ, Robinson, HL, Haigwood, NL & Montefiori, DC 1999, 'Neutralizing antibody responses in Africa green monkeys naturally infected with simian immunodeficiency virus (SIVagm)', Journal of Medical Primatology, vol. 28, no. 3, pp. 97-104.
Gicheru, Michael M. ; Otsyula, Moses ; Spearman, Paul ; Graham, Barney S. ; Miller, Chris J ; Robinson, Harriet L. ; Haigwood, Nancy L. ; Montefiori, David C. / Neutralizing antibody responses in Africa green monkeys naturally infected with simian immunodeficiency virus (SIVagm). In: Journal of Medical Primatology. 1999 ; Vol. 28, No. 3. pp. 97-104.
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