Neutralization profiles of sera from human immunodeficiency virus (HIV)- infected individuals: Relationship to HIV viral load and CD4 cell count

Mostafa Nokta, Patricia Turk, Kimberly Loesch, Richard B Pollard

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The relationship of the neutralizing activity (NA) profile of sera from human immunodeficiency virus (HIV)-infected individuals to the HIV viral load and the absolute CD4 count was examined. The NA of 24 serum samples against autologous isolates (AI) and HIV type 1 strain MN was examined. Three NA patterns were recognized. Nine sera neutralized both AI and MN (+/+), six sera neutralized MN but not AI (-/+), and nine sera failed to neutralize both AI and MN (-/-). The identification of the three neutralization patterns (+/+, -/+, and -/-) indicated that resistance to neutralization was progressive. A reciprocal relationship between the viral burden of the patients and the NA profiles was observed. The nine subjects with a -/- NA profile had a plasma viral load of ≥5 x 104 copies/ml and a cellular viral burden of ≥1,122 infectious units per million viable cells, which were significantly different from those of the other groups (P < 0.02). These patterns were independent of the phenotypic characteristics of the virus. Longitudinally, subjects with a -/- profile at baseline gained their HIV- specific NA by 24 weeks of antiretroviral therapy when this was associated with a ≥1-log10 decline in the plasma HIV viral load. The sera from week 24 from some patients were able to neutralize both the 24-week and the baseline dominant virus isolates. A change in CD4 cell count of 50 or more in either direction predicted a -/- or +/+ profile. The verification of the autologous NA profile might be important in selecting patients who may benefit from immune-based therapies involving neutralizing monoclonal antibodies.

Original languageEnglish (US)
Pages (from-to)412-416
Number of pages5
JournalClinical and Diagnostic Laboratory Immunology
Volume7
Issue number3
DOIs
StatePublished - May 2000
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology (medical)
  • Immunology and Allergy
  • Clinical Biochemistry
  • Immunology

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