Abstract
Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3940-3949 |
| Number of pages | 10 |
| Journal | Journal of Dairy Science |
| Volume | 93 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2010 |
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Keywords
- High performance liquid chromatography
- Holstein-Friesian colostrum
- Oligosaccharide
- Time-of-flight mass spectrometry
ASJC Scopus subject areas
- Animal Science and Zoology
- Food Science
- Genetics
Cite this
Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry. / Barile, D.; Marotta, M.; Chu, C.; Mehra, R.; Grimm, R.; Lebrilla, Carlito B; German, J. B.
In: Journal of Dairy Science, Vol. 93, No. 9, 09.2010, p. 3940-3949.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry
AU - Barile, D.
AU - Marotta, M.
AU - Chu, C.
AU - Mehra, R.
AU - Grimm, R.
AU - Lebrilla, Carlito B
AU - German, J. B.
PY - 2010/9
Y1 - 2010/9
N2 - Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.
AB - Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.
KW - High performance liquid chromatography
KW - Holstein-Friesian colostrum
KW - Oligosaccharide
KW - Time-of-flight mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=77955652012&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955652012&partnerID=8YFLogxK
U2 - 10.3168/jds.2010-3156
DO - 10.3168/jds.2010-3156
M3 - Article
C2 - 20723667
AN - SCOPUS:77955652012
VL - 93
SP - 3940
EP - 3949
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 9
ER -