Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry

D. Barile, M. Marotta, C. Chu, R. Mehra, R. Grimm, Carlito B Lebrilla, J. B. German

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.

Original languageEnglish (US)
Pages (from-to)3940-3949
Number of pages10
JournalJournal of Dairy Science
Volume93
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

Colostrum
Microfluidics
colostrum
Oligosaccharides
Lactation
oligosaccharides
Mass Spectrometry
Holstein
lactation
high performance liquid chromatography
High Pressure Liquid Chromatography
mass spectrometry
Milk
cows
milk
cow colostrum
lactation number
Food Industry
Drug Industry
Insurance Benefits

Keywords

  • High performance liquid chromatography
  • Holstein-Friesian colostrum
  • Oligosaccharide
  • Time-of-flight mass spectrometry

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Food Science
  • Genetics

Cite this

Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry. / Barile, D.; Marotta, M.; Chu, C.; Mehra, R.; Grimm, R.; Lebrilla, Carlito B; German, J. B.

In: Journal of Dairy Science, Vol. 93, No. 9, 09.2010, p. 3940-3949.

Research output: Contribution to journalArticle

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