Neurovirulence of the UC-2 and UC-8 Strains of Bluetongue Virus Serotype 11 in Newborn Mice

A. S. Waldvogel, C. A. Anderson, Robert Higgins, Bennie Osburn

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In vivo and in vitro experiments were done to investigate whether the difference in neurovirulence between the two strains of bluetongue virus 11, UC-2 and UC-8, is based on a different capability to gain access to the brain from the subcutaneous inoculation site or on a different tropism for neural cells. In newborn Balb/c mice subcutaneous inoculation of UC-8 at doses between 10−0.2 plaque forming units (PFU) and 104.8 PFU caused a severe necrotizing encephalitis whereas UC-2 at doses of up to 104.4 PFU did not affect newborn Balb/c mice. However, intracranial inoculation of 102.4 PFU of either virus strain produced severe necrotizing encephalitis. In vitro both virus strains infected dissociated brain cell cultures similarly. Double labelling immunofluorescent staining with markers specific for neural cells did not reveal differences in the target cells for the two viruses. The difference in neurovirulence between UC-2 and UC-8, therefore, appears to be determined by the ability of UC-8 to infect the brain from a subcutaneous inoculation site.

Original languageEnglish (US)
Pages (from-to)404-410
Number of pages7
JournalVeterinary Pathology
Volume24
Issue number5
DOIs
StatePublished - Jan 1 1987

Fingerprint

Bluetongue virus
neonates
serotypes
Encephalitis
encephalitis
Viruses
brain
viruses
mice
Brain
neurons
fluorescent labeling
tropisms
Tropism
dosage
cell culture
Cell Culture Techniques
Staining and Labeling
Serogroup
cells

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Neurovirulence of the UC-2 and UC-8 Strains of Bluetongue Virus Serotype 11 in Newborn Mice. / Waldvogel, A. S.; Anderson, C. A.; Higgins, Robert; Osburn, Bennie.

In: Veterinary Pathology, Vol. 24, No. 5, 01.01.1987, p. 404-410.

Research output: Contribution to journalArticle

Waldvogel, A. S. ; Anderson, C. A. ; Higgins, Robert ; Osburn, Bennie. / Neurovirulence of the UC-2 and UC-8 Strains of Bluetongue Virus Serotype 11 in Newborn Mice. In: Veterinary Pathology. 1987 ; Vol. 24, No. 5. pp. 404-410.
@article{282cf9d363f345d580cb733b2b750123,
title = "Neurovirulence of the UC-2 and UC-8 Strains of Bluetongue Virus Serotype 11 in Newborn Mice",
abstract = "In vivo and in vitro experiments were done to investigate whether the difference in neurovirulence between the two strains of bluetongue virus 11, UC-2 and UC-8, is based on a different capability to gain access to the brain from the subcutaneous inoculation site or on a different tropism for neural cells. In newborn Balb/c mice subcutaneous inoculation of UC-8 at doses between 10−0.2 plaque forming units (PFU) and 104.8 PFU caused a severe necrotizing encephalitis whereas UC-2 at doses of up to 104.4 PFU did not affect newborn Balb/c mice. However, intracranial inoculation of 102.4 PFU of either virus strain produced severe necrotizing encephalitis. In vitro both virus strains infected dissociated brain cell cultures similarly. Double labelling immunofluorescent staining with markers specific for neural cells did not reveal differences in the target cells for the two viruses. The difference in neurovirulence between UC-2 and UC-8, therefore, appears to be determined by the ability of UC-8 to infect the brain from a subcutaneous inoculation site.",
author = "Waldvogel, {A. S.} and Anderson, {C. A.} and Robert Higgins and Bennie Osburn",
year = "1987",
month = "1",
day = "1",
doi = "10.1177/030098588702400507",
language = "English (US)",
volume = "24",
pages = "404--410",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "5",

}

TY - JOUR

T1 - Neurovirulence of the UC-2 and UC-8 Strains of Bluetongue Virus Serotype 11 in Newborn Mice

AU - Waldvogel, A. S.

AU - Anderson, C. A.

AU - Higgins, Robert

AU - Osburn, Bennie

PY - 1987/1/1

Y1 - 1987/1/1

N2 - In vivo and in vitro experiments were done to investigate whether the difference in neurovirulence between the two strains of bluetongue virus 11, UC-2 and UC-8, is based on a different capability to gain access to the brain from the subcutaneous inoculation site or on a different tropism for neural cells. In newborn Balb/c mice subcutaneous inoculation of UC-8 at doses between 10−0.2 plaque forming units (PFU) and 104.8 PFU caused a severe necrotizing encephalitis whereas UC-2 at doses of up to 104.4 PFU did not affect newborn Balb/c mice. However, intracranial inoculation of 102.4 PFU of either virus strain produced severe necrotizing encephalitis. In vitro both virus strains infected dissociated brain cell cultures similarly. Double labelling immunofluorescent staining with markers specific for neural cells did not reveal differences in the target cells for the two viruses. The difference in neurovirulence between UC-2 and UC-8, therefore, appears to be determined by the ability of UC-8 to infect the brain from a subcutaneous inoculation site.

AB - In vivo and in vitro experiments were done to investigate whether the difference in neurovirulence between the two strains of bluetongue virus 11, UC-2 and UC-8, is based on a different capability to gain access to the brain from the subcutaneous inoculation site or on a different tropism for neural cells. In newborn Balb/c mice subcutaneous inoculation of UC-8 at doses between 10−0.2 plaque forming units (PFU) and 104.8 PFU caused a severe necrotizing encephalitis whereas UC-2 at doses of up to 104.4 PFU did not affect newborn Balb/c mice. However, intracranial inoculation of 102.4 PFU of either virus strain produced severe necrotizing encephalitis. In vitro both virus strains infected dissociated brain cell cultures similarly. Double labelling immunofluorescent staining with markers specific for neural cells did not reveal differences in the target cells for the two viruses. The difference in neurovirulence between UC-2 and UC-8, therefore, appears to be determined by the ability of UC-8 to infect the brain from a subcutaneous inoculation site.

UR - http://www.scopus.com/inward/record.url?scp=0023409261&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023409261&partnerID=8YFLogxK

U2 - 10.1177/030098588702400507

DO - 10.1177/030098588702400507

M3 - Article

C2 - 2823445

AN - SCOPUS:0023409261

VL - 24

SP - 404

EP - 410

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 5

ER -