Abstract
The allosteric modulation of GABA(A) receptors in the rat brain cortex by neurosteroids was studied at different developmental stages. GABA(A) receptors were identified using [3H]muscimol binding to membrane preparations obtained from embryos and neonates (postnatal day 0-PN0; postnatal day 5-PN5). Data analysis disclosed a unique population of binding sites at all ages tested. An increase in the number of receptors was observed during development reaching almost adult levels at PN5. The neurosteroids pregnanolone and allopregnanolone failed to modulate [3H]muscimol specific binding in embryos and neonates, but a positive modulation was obtained in 5-day old animals. The addition of 1 μM pregnanolone induced a 3-6-fold increase [3H]muscimol affinity in PN5 (n = 3; P < 0.03), and a 2-fold increase in receptors number in adults (n = 3; P < 0.03). The differences observed in allosteric modulation during development suggest that a change occurred during the first week of life, and this change might affect GABA(A) receptor function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 313-317 |
| Number of pages | 5 |
| Journal | Neurochemistry International |
| Volume | 31 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jun 7 1997 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Cellular and Molecular Neuroscience
Cite this
Neurosteroid modulation of GABA(A) receptors in the developing rat brain cortex. / Borodinsky, Laura N; Pesce, Guido; Pomata, Pablo; Fiszman, Mónica L.
In: Neurochemistry International, Vol. 31, No. 2, 07.06.1997, p. 313-317.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neurosteroid modulation of GABA(A) receptors in the developing rat brain cortex
AU - Borodinsky, Laura N
AU - Pesce, Guido
AU - Pomata, Pablo
AU - Fiszman, Mónica L.
PY - 1997/6/7
Y1 - 1997/6/7
N2 - The allosteric modulation of GABA(A) receptors in the rat brain cortex by neurosteroids was studied at different developmental stages. GABA(A) receptors were identified using [3H]muscimol binding to membrane preparations obtained from embryos and neonates (postnatal day 0-PN0; postnatal day 5-PN5). Data analysis disclosed a unique population of binding sites at all ages tested. An increase in the number of receptors was observed during development reaching almost adult levels at PN5. The neurosteroids pregnanolone and allopregnanolone failed to modulate [3H]muscimol specific binding in embryos and neonates, but a positive modulation was obtained in 5-day old animals. The addition of 1 μM pregnanolone induced a 3-6-fold increase [3H]muscimol affinity in PN5 (n = 3; P < 0.03), and a 2-fold increase in receptors number in adults (n = 3; P < 0.03). The differences observed in allosteric modulation during development suggest that a change occurred during the first week of life, and this change might affect GABA(A) receptor function.
AB - The allosteric modulation of GABA(A) receptors in the rat brain cortex by neurosteroids was studied at different developmental stages. GABA(A) receptors were identified using [3H]muscimol binding to membrane preparations obtained from embryos and neonates (postnatal day 0-PN0; postnatal day 5-PN5). Data analysis disclosed a unique population of binding sites at all ages tested. An increase in the number of receptors was observed during development reaching almost adult levels at PN5. The neurosteroids pregnanolone and allopregnanolone failed to modulate [3H]muscimol specific binding in embryos and neonates, but a positive modulation was obtained in 5-day old animals. The addition of 1 μM pregnanolone induced a 3-6-fold increase [3H]muscimol affinity in PN5 (n = 3; P < 0.03), and a 2-fold increase in receptors number in adults (n = 3; P < 0.03). The differences observed in allosteric modulation during development suggest that a change occurred during the first week of life, and this change might affect GABA(A) receptor function.
UR - http://www.scopus.com/inward/record.url?scp=0030610503&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030610503&partnerID=8YFLogxK
U2 - 10.1016/S0197-0186(96)00139-8
DO - 10.1016/S0197-0186(96)00139-8
M3 - Article
C2 - 9220465
AN - SCOPUS:0030610503
VL - 31
SP - 313
EP - 317
JO - Neurochemistry International
JF - Neurochemistry International
SN - 0197-0186
IS - 2
ER -