Neurosteroid modulation of GABA(A) receptors in the developing rat brain cortex

Laura N Borodinsky, Guido Pesce, Pablo Pomata, Mónica L. Fiszman

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The allosteric modulation of GABA(A) receptors in the rat brain cortex by neurosteroids was studied at different developmental stages. GABA(A) receptors were identified using [3H]muscimol binding to membrane preparations obtained from embryos and neonates (postnatal day 0-PN0; postnatal day 5-PN5). Data analysis disclosed a unique population of binding sites at all ages tested. An increase in the number of receptors was observed during development reaching almost adult levels at PN5. The neurosteroids pregnanolone and allopregnanolone failed to modulate [3H]muscimol specific binding in embryos and neonates, but a positive modulation was obtained in 5-day old animals. The addition of 1 μM pregnanolone induced a 3-6-fold increase [3H]muscimol affinity in PN5 (n = 3; P < 0.03), and a 2-fold increase in receptors number in adults (n = 3; P < 0.03). The differences observed in allosteric modulation during development suggest that a change occurred during the first week of life, and this change might affect GABA(A) receptor function.

Original languageEnglish (US)
Pages (from-to)313-317
Number of pages5
JournalNeurochemistry International
Volume31
Issue number2
DOIs
StatePublished - Jun 7 1997
Externally publishedYes

Fingerprint

Pregnanolone
Muscimol
GABA-A Receptors
Neurotransmitter Agents
Brain
Embryonic Structures
Binding Sites
Membranes
Population

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Neurosteroid modulation of GABA(A) receptors in the developing rat brain cortex. / Borodinsky, Laura N; Pesce, Guido; Pomata, Pablo; Fiszman, Mónica L.

In: Neurochemistry International, Vol. 31, No. 2, 07.06.1997, p. 313-317.

Research output: Contribution to journalArticle

Borodinsky, Laura N ; Pesce, Guido ; Pomata, Pablo ; Fiszman, Mónica L. / Neurosteroid modulation of GABA(A) receptors in the developing rat brain cortex. In: Neurochemistry International. 1997 ; Vol. 31, No. 2. pp. 313-317.
@article{f9ab32aba49d41bc8c1d57d13bb8afc5,
title = "Neurosteroid modulation of GABA(A) receptors in the developing rat brain cortex",
abstract = "The allosteric modulation of GABA(A) receptors in the rat brain cortex by neurosteroids was studied at different developmental stages. GABA(A) receptors were identified using [3H]muscimol binding to membrane preparations obtained from embryos and neonates (postnatal day 0-PN0; postnatal day 5-PN5). Data analysis disclosed a unique population of binding sites at all ages tested. An increase in the number of receptors was observed during development reaching almost adult levels at PN5. The neurosteroids pregnanolone and allopregnanolone failed to modulate [3H]muscimol specific binding in embryos and neonates, but a positive modulation was obtained in 5-day old animals. The addition of 1 μM pregnanolone induced a 3-6-fold increase [3H]muscimol affinity in PN5 (n = 3; P < 0.03), and a 2-fold increase in receptors number in adults (n = 3; P < 0.03). The differences observed in allosteric modulation during development suggest that a change occurred during the first week of life, and this change might affect GABA(A) receptor function.",
author = "Borodinsky, {Laura N} and Guido Pesce and Pablo Pomata and Fiszman, {M{\'o}nica L.}",
year = "1997",
month = "6",
day = "7",
doi = "10.1016/S0197-0186(96)00139-8",
language = "English (US)",
volume = "31",
pages = "313--317",
journal = "Neurochemistry International",
issn = "0197-0186",
publisher = "Elsevier Limited",
number = "2",

}

TY - JOUR

T1 - Neurosteroid modulation of GABA(A) receptors in the developing rat brain cortex

AU - Borodinsky, Laura N

AU - Pesce, Guido

AU - Pomata, Pablo

AU - Fiszman, Mónica L.

PY - 1997/6/7

Y1 - 1997/6/7

N2 - The allosteric modulation of GABA(A) receptors in the rat brain cortex by neurosteroids was studied at different developmental stages. GABA(A) receptors were identified using [3H]muscimol binding to membrane preparations obtained from embryos and neonates (postnatal day 0-PN0; postnatal day 5-PN5). Data analysis disclosed a unique population of binding sites at all ages tested. An increase in the number of receptors was observed during development reaching almost adult levels at PN5. The neurosteroids pregnanolone and allopregnanolone failed to modulate [3H]muscimol specific binding in embryos and neonates, but a positive modulation was obtained in 5-day old animals. The addition of 1 μM pregnanolone induced a 3-6-fold increase [3H]muscimol affinity in PN5 (n = 3; P < 0.03), and a 2-fold increase in receptors number in adults (n = 3; P < 0.03). The differences observed in allosteric modulation during development suggest that a change occurred during the first week of life, and this change might affect GABA(A) receptor function.

AB - The allosteric modulation of GABA(A) receptors in the rat brain cortex by neurosteroids was studied at different developmental stages. GABA(A) receptors were identified using [3H]muscimol binding to membrane preparations obtained from embryos and neonates (postnatal day 0-PN0; postnatal day 5-PN5). Data analysis disclosed a unique population of binding sites at all ages tested. An increase in the number of receptors was observed during development reaching almost adult levels at PN5. The neurosteroids pregnanolone and allopregnanolone failed to modulate [3H]muscimol specific binding in embryos and neonates, but a positive modulation was obtained in 5-day old animals. The addition of 1 μM pregnanolone induced a 3-6-fold increase [3H]muscimol affinity in PN5 (n = 3; P < 0.03), and a 2-fold increase in receptors number in adults (n = 3; P < 0.03). The differences observed in allosteric modulation during development suggest that a change occurred during the first week of life, and this change might affect GABA(A) receptor function.

UR - http://www.scopus.com/inward/record.url?scp=0030610503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030610503&partnerID=8YFLogxK

U2 - 10.1016/S0197-0186(96)00139-8

DO - 10.1016/S0197-0186(96)00139-8

M3 - Article

C2 - 9220465

AN - SCOPUS:0030610503

VL - 31

SP - 313

EP - 317

JO - Neurochemistry International

JF - Neurochemistry International

SN - 0197-0186

IS - 2

ER -