Abstract
1. The effects of paeoniflorin (PF), a compound isolated from Paeony radix, on neurological impairment and histologically measured infarction volume following transient and permanent focal ischemia were examined in Sprague-Dawley rats. 2. In transient ischemia model, rats were subjected to a 1.5-h occlusion of the middle cerebral artery (MCA). The administration of PF (2.5 and 5mg kg -1, s.c.) produced a dose-dependent decrease in both neurological impairment and the histologically measured infarction volume. Similar results were also obtained when PF (2.5, 5, and 10mg kg -1, s.c.) was given in permanent ischemia model. 3. The neuroprotective effect of PF (10mg kg -1, s.c.) was abolished by pretreatment of DPCPX (0.25mg kg -1, s.c.), a selective adenosine A 1 receptor (A 1R) antagonist. 4. PF (10, 40, and 160mg kg -1, i.v.) had no effect on mean arterial pressure (MAP) and heart rates (HR) in the conscious rat. Additionally, PF (10 -3 mol l -1) had no effect on noradrenaline- (NA-) or high K + concentration-induced contractions of isolated rabbit primary artery. 5. In competitive binding experiments, PF did not compete with the binding of [ 3H]DPCPX, but displaced the binding of [ 3H]NECA to the membrane preparation of rat cerebral cortex. This binding manner was distinguished from the classical A 1R agonists. 6. The results demonstrated that activation of A 1R might be involved in PF-induced neuroprotection in cerebral ischemia in rat. However, PF had no 'well-known' cardiovascular side effects of classical A 1R agonists. The results suggest that PF might have the potential therapeutic value as an anti-stroke drug.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 604-611 |
| Number of pages | 8 |
| Journal | British Journal of Pharmacology |
| Volume | 146 |
| Issue number | 4 |
| DOIs | |
| State | Published - Oct 1 2005 |
| Externally published | Yes |
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Keywords
- Adenosine A receptor
- Cardiovascular side effect
- Neuroprotective effect
- Paeoniflorin
- Permanent cerebral ischemia
- Transient cerebral ischemia
ASJC Scopus subject areas
- Pharmacology
Cite this
Neuroprotective effect of paeoniflorin on cerebral ischemic rat by activating adenosine A 1 receptor in a manner different from its classical agonists. / Liu, Da; Xie, Ke Qiang; Ji, Xin Quan; Ye, Yang; Jiang, Cheng Liang; Zhu, Xing Zu.
In: British Journal of Pharmacology, Vol. 146, No. 4, 01.10.2005, p. 604-611.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neuroprotective effect of paeoniflorin on cerebral ischemic rat by activating adenosine A 1 receptor in a manner different from its classical agonists
AU - Liu, Da
AU - Xie, Ke Qiang
AU - Ji, Xin Quan
AU - Ye, Yang
AU - Jiang, Cheng Liang
AU - Zhu, Xing Zu
PY - 2005/10/1
Y1 - 2005/10/1
N2 - 1. The effects of paeoniflorin (PF), a compound isolated from Paeony radix, on neurological impairment and histologically measured infarction volume following transient and permanent focal ischemia were examined in Sprague-Dawley rats. 2. In transient ischemia model, rats were subjected to a 1.5-h occlusion of the middle cerebral artery (MCA). The administration of PF (2.5 and 5mg kg -1, s.c.) produced a dose-dependent decrease in both neurological impairment and the histologically measured infarction volume. Similar results were also obtained when PF (2.5, 5, and 10mg kg -1, s.c.) was given in permanent ischemia model. 3. The neuroprotective effect of PF (10mg kg -1, s.c.) was abolished by pretreatment of DPCPX (0.25mg kg -1, s.c.), a selective adenosine A 1 receptor (A 1R) antagonist. 4. PF (10, 40, and 160mg kg -1, i.v.) had no effect on mean arterial pressure (MAP) and heart rates (HR) in the conscious rat. Additionally, PF (10 -3 mol l -1) had no effect on noradrenaline- (NA-) or high K + concentration-induced contractions of isolated rabbit primary artery. 5. In competitive binding experiments, PF did not compete with the binding of [ 3H]DPCPX, but displaced the binding of [ 3H]NECA to the membrane preparation of rat cerebral cortex. This binding manner was distinguished from the classical A 1R agonists. 6. The results demonstrated that activation of A 1R might be involved in PF-induced neuroprotection in cerebral ischemia in rat. However, PF had no 'well-known' cardiovascular side effects of classical A 1R agonists. The results suggest that PF might have the potential therapeutic value as an anti-stroke drug.
AB - 1. The effects of paeoniflorin (PF), a compound isolated from Paeony radix, on neurological impairment and histologically measured infarction volume following transient and permanent focal ischemia were examined in Sprague-Dawley rats. 2. In transient ischemia model, rats were subjected to a 1.5-h occlusion of the middle cerebral artery (MCA). The administration of PF (2.5 and 5mg kg -1, s.c.) produced a dose-dependent decrease in both neurological impairment and the histologically measured infarction volume. Similar results were also obtained when PF (2.5, 5, and 10mg kg -1, s.c.) was given in permanent ischemia model. 3. The neuroprotective effect of PF (10mg kg -1, s.c.) was abolished by pretreatment of DPCPX (0.25mg kg -1, s.c.), a selective adenosine A 1 receptor (A 1R) antagonist. 4. PF (10, 40, and 160mg kg -1, i.v.) had no effect on mean arterial pressure (MAP) and heart rates (HR) in the conscious rat. Additionally, PF (10 -3 mol l -1) had no effect on noradrenaline- (NA-) or high K + concentration-induced contractions of isolated rabbit primary artery. 5. In competitive binding experiments, PF did not compete with the binding of [ 3H]DPCPX, but displaced the binding of [ 3H]NECA to the membrane preparation of rat cerebral cortex. This binding manner was distinguished from the classical A 1R agonists. 6. The results demonstrated that activation of A 1R might be involved in PF-induced neuroprotection in cerebral ischemia in rat. However, PF had no 'well-known' cardiovascular side effects of classical A 1R agonists. The results suggest that PF might have the potential therapeutic value as an anti-stroke drug.
KW - Adenosine A receptor
KW - Cardiovascular side effect
KW - Neuroprotective effect
KW - Paeoniflorin
KW - Permanent cerebral ischemia
KW - Transient cerebral ischemia
UR - http://www.scopus.com/inward/record.url?scp=27144558321&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27144558321&partnerID=8YFLogxK
U2 - 10.1038/sj.bjp.0706335
DO - 10.1038/sj.bjp.0706335
M3 - Article
C2 - 16086036
AN - SCOPUS:27144558321
VL - 146
SP - 604
EP - 611
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
SN - 0007-1188
IS - 4
ER -