Neurons derived from radial glial cells establish radial units in neocortex

Stephen C Noctor, Alexander C. Flint, Tamily A. Weissman, Ryan S. Dammerman, Arnold R. Kriegstein

Research output: Contribution to journalArticle

1332 Citations (Scopus)

Abstract

The neocortex of the adult brain consists of neurons and glia that are generated by precursor cells of the embryonic ventricular zone. In general, glia are generated after neurons during development1, but radial glia are an exception to this rule. Radial glia are generated before neurogenesis and guide neuronal migration2. Radial glia are mitotically active throughout neurogenesis3, and disappear or become astrocytes when neuronal migration is complete4,5. Although the lineage relationships of cortical neurons and glia have been explored6,7, the clonal relationship of radial glia to other cortical cells remains unknown. It has been suggested that radial glia may be neuronal precursors5,8-10, but this has not been demonstrated in vivo. We have used a retroviral vector encoding enhanced green fluorescent protein to label precursor cells in vivo and have examined clones 1-3 days later using morphological, immunohistochemical and electrophysiological techniques. Here we show that clones consist of mitotic radial glia and postmitotic neurons, and that neurons migrate along clonally related radial glia. Time-lapse images show that proliferative radial glia generate neurons. Our results support the concept that a lineage relationship between neurons and proliferative radial glia may underlie the radial organization of neocortex.

Original languageEnglish (US)
Pages (from-to)714-720
Number of pages7
JournalNature
Volume409
Issue number6821
DOIs
StatePublished - Feb 8 2001
Externally publishedYes

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Ependymoglial Cells
Neocortex
Neuroglia
Neurons
Clone Cells
Neurogenesis
Astrocytes

ASJC Scopus subject areas

  • General

Cite this

Noctor, S. C., Flint, A. C., Weissman, T. A., Dammerman, R. S., & Kriegstein, A. R. (2001). Neurons derived from radial glial cells establish radial units in neocortex. Nature, 409(6821), 714-720. https://doi.org/10.1038/35055553

Neurons derived from radial glial cells establish radial units in neocortex. / Noctor, Stephen C; Flint, Alexander C.; Weissman, Tamily A.; Dammerman, Ryan S.; Kriegstein, Arnold R.

In: Nature, Vol. 409, No. 6821, 08.02.2001, p. 714-720.

Research output: Contribution to journalArticle

Noctor, SC, Flint, AC, Weissman, TA, Dammerman, RS & Kriegstein, AR 2001, 'Neurons derived from radial glial cells establish radial units in neocortex', Nature, vol. 409, no. 6821, pp. 714-720. https://doi.org/10.1038/35055553
Noctor SC, Flint AC, Weissman TA, Dammerman RS, Kriegstein AR. Neurons derived from radial glial cells establish radial units in neocortex. Nature. 2001 Feb 8;409(6821):714-720. https://doi.org/10.1038/35055553
Noctor, Stephen C ; Flint, Alexander C. ; Weissman, Tamily A. ; Dammerman, Ryan S. ; Kriegstein, Arnold R. / Neurons derived from radial glial cells establish radial units in neocortex. In: Nature. 2001 ; Vol. 409, No. 6821. pp. 714-720.
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