Neuronal transfection in brain slices using particle-mediated gene transfer

Donald C. Lo, A Kimberley Usrey, Lawrence C. Katz

Research output: Contribution to journalArticle

196 Citations (Scopus)

Abstract

Difficulties in neuronal transfection continue to restrict the applicability of molecular approaches to neurobiology. Conventional transfection techniques have been of limited effectiveness, particularly in intact neural tissues. Viral vectors effectively transfect neurons both in vitro and in vivo but are labor intensive to construct, difficult to control, and often compromise cell viability. We describe here an alternative strategy using particle-mediated gene transfer for the transfection of neurons and glia in intact brain slices. This approach is efficient, reliable, and does not require advanced molecular biological facilities for its application.

Original languageEnglish (US)
Pages (from-to)1263-1268
Number of pages6
JournalNeuron
Volume13
Issue number6
DOIs
StatePublished - 1994
Externally publishedYes

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Transfection
Brain
Genes
Neurons
Neurobiology
Neuroglia
Cell Survival
In Vitro Techniques

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuronal transfection in brain slices using particle-mediated gene transfer. / Lo, Donald C.; Usrey, A Kimberley; Katz, Lawrence C.

In: Neuron, Vol. 13, No. 6, 1994, p. 1263-1268.

Research output: Contribution to journalArticle

Lo, Donald C. ; Usrey, A Kimberley ; Katz, Lawrence C. / Neuronal transfection in brain slices using particle-mediated gene transfer. In: Neuron. 1994 ; Vol. 13, No. 6. pp. 1263-1268.
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