Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats

V. H. Routh, D. M. Murakami, J. S. Stern, C. A. Fuller, Barbara A Horwitz

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Central neural activity was assessed by measuring relative cytochrome oxidase (CO) activity in the ventromedial nucleus (VMN; thermogenesis regulation), the parvocellular paraventricular nucleus (PVN; feeding regulation), and the magnocellular PVN (secretion of vasopressin and oxytocin) in 10 age-matched pairs of 39- to 42-day-old Zucker rats. When obese (fa/fa) were compared to lean (Fa/Fa) rats, relative CO activity was significantly lower (~ 10 percent) in the VMN and parvocellular PVN, but not in the magnocellular PVN. Cell diameters did not differ. To determine if there were corresponding differences in levels or release of hypothalamic monoamines, we compared 7 pairs of 90- to 94-day-old lean (Fa/?) and obese (fa/fa) rats at rest and after 2 h of 9 °C. Tissue punches from frozen PVN, VMN, and preoptic area (the latter being a site of thermosensitive units modulating VMN output) were assayed. In obese vs. lean noncold-exposed rats, we observed lower concentrations of: 5-hydroxyindoleacetic acid (5HIAA; metabolite of serotonin, 5HT) in the VMN; 3-methoxy-4-hydroxyphenylglycol (MHPG; metabolite of norepinephrine, NE) and NE + MHPG (index of total NE) in the preoptic area; and 3,4-dihydroxyphenylacetic acid (DOPAC; metabolite of dopamine, DA) in the PVN. Additionally, in the VMN, cold exposure resulted in: elevated concentrations of MHPG and MHPG + NE in both lean and obese rats; elevated concentrations of 5HT, 5HIAA, and 5HT + 5HIAA in obese rats, with no significant changes in these variables in lean animals; decreased ratio of 5HIAA/5HT in obese rats and increased rats in leans. In the preoptic region, cold exposure led to increased concentrations of MHPG, NE + MHPG, 5HT, and 5HT + 5HIAA in obese but not lean rats. In the PVN, 5HT concentrations were increased in cold-exposed obese but not lean rats. Our data support the hypothesis that neuronal activity in obese rats differs from that of lean rats at rest and during cold exposure and suggest the several monoamine systems play a role in such differences.

Original languageEnglish (US)
Pages (from-to)879-891
Number of pages13
JournalInternational Journal of Obesity
Volume14
Issue number10
StatePublished - 1990

Fingerprint

Zucker Rats
Methoxyhydroxyphenylglycol
rats
Norepinephrine
3,4-Dihydroxyphenylacetic Acid
preoptic area
Preoptic Area
monoamines
Electron Transport Complex IV
metabolites
cytochrome-c oxidase
5-hydroxyindoleacetic acid
Hydroxyindoleacetic Acid
Thermogenesis
Paraventricular Hypothalamic Nucleus
vasopressin
Oxytocin
oxytocin
cold zones
Vasopressins

Keywords

  • brown fat
  • cytochrome oxidase
  • hypothalamic monoamines
  • hypothalamic norepinephrine turnover
  • hypothalamic serotonin turnover
  • obesity
  • paraventricular nucleus
  • preoptic area
  • ventromedial nucleus
  • Zucker rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Public Health, Environmental and Occupational Health
  • Endocrinology
  • Food Science
  • Endocrinology, Diabetes and Metabolism

Cite this

Routh, V. H., Murakami, D. M., Stern, J. S., Fuller, C. A., & Horwitz, B. A. (1990). Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats. International Journal of Obesity, 14(10), 879-891.

Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats. / Routh, V. H.; Murakami, D. M.; Stern, J. S.; Fuller, C. A.; Horwitz, Barbara A.

In: International Journal of Obesity, Vol. 14, No. 10, 1990, p. 879-891.

Research output: Contribution to journalArticle

Routh, VH, Murakami, DM, Stern, JS, Fuller, CA & Horwitz, BA 1990, 'Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats', International Journal of Obesity, vol. 14, no. 10, pp. 879-891.
Routh VH, Murakami DM, Stern JS, Fuller CA, Horwitz BA. Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats. International Journal of Obesity. 1990;14(10):879-891.
Routh, V. H. ; Murakami, D. M. ; Stern, J. S. ; Fuller, C. A. ; Horwitz, Barbara A. / Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats. In: International Journal of Obesity. 1990 ; Vol. 14, No. 10. pp. 879-891.
@article{19bb6a90413244778ac45465c7d4787e,
title = "Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats",
abstract = "Central neural activity was assessed by measuring relative cytochrome oxidase (CO) activity in the ventromedial nucleus (VMN; thermogenesis regulation), the parvocellular paraventricular nucleus (PVN; feeding regulation), and the magnocellular PVN (secretion of vasopressin and oxytocin) in 10 age-matched pairs of 39- to 42-day-old Zucker rats. When obese (fa/fa) were compared to lean (Fa/Fa) rats, relative CO activity was significantly lower (~ 10 percent) in the VMN and parvocellular PVN, but not in the magnocellular PVN. Cell diameters did not differ. To determine if there were corresponding differences in levels or release of hypothalamic monoamines, we compared 7 pairs of 90- to 94-day-old lean (Fa/?) and obese (fa/fa) rats at rest and after 2 h of 9 °C. Tissue punches from frozen PVN, VMN, and preoptic area (the latter being a site of thermosensitive units modulating VMN output) were assayed. In obese vs. lean noncold-exposed rats, we observed lower concentrations of: 5-hydroxyindoleacetic acid (5HIAA; metabolite of serotonin, 5HT) in the VMN; 3-methoxy-4-hydroxyphenylglycol (MHPG; metabolite of norepinephrine, NE) and NE + MHPG (index of total NE) in the preoptic area; and 3,4-dihydroxyphenylacetic acid (DOPAC; metabolite of dopamine, DA) in the PVN. Additionally, in the VMN, cold exposure resulted in: elevated concentrations of MHPG and MHPG + NE in both lean and obese rats; elevated concentrations of 5HT, 5HIAA, and 5HT + 5HIAA in obese rats, with no significant changes in these variables in lean animals; decreased ratio of 5HIAA/5HT in obese rats and increased rats in leans. In the preoptic region, cold exposure led to increased concentrations of MHPG, NE + MHPG, 5HT, and 5HT + 5HIAA in obese but not lean rats. In the PVN, 5HT concentrations were increased in cold-exposed obese but not lean rats. Our data support the hypothesis that neuronal activity in obese rats differs from that of lean rats at rest and during cold exposure and suggest the several monoamine systems play a role in such differences.",
keywords = "brown fat, cytochrome oxidase, hypothalamic monoamines, hypothalamic norepinephrine turnover, hypothalamic serotonin turnover, obesity, paraventricular nucleus, preoptic area, ventromedial nucleus, Zucker rats",
author = "Routh, {V. H.} and Murakami, {D. M.} and Stern, {J. S.} and Fuller, {C. A.} and Horwitz, {Barbara A}",
year = "1990",
language = "English (US)",
volume = "14",
pages = "879--891",
journal = "International Journal of Obesity",
issn = "0307-0565",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Neuronal activity in hypothalamic nuclei of obese and lean Zucker rats

AU - Routh, V. H.

AU - Murakami, D. M.

AU - Stern, J. S.

AU - Fuller, C. A.

AU - Horwitz, Barbara A

PY - 1990

Y1 - 1990

N2 - Central neural activity was assessed by measuring relative cytochrome oxidase (CO) activity in the ventromedial nucleus (VMN; thermogenesis regulation), the parvocellular paraventricular nucleus (PVN; feeding regulation), and the magnocellular PVN (secretion of vasopressin and oxytocin) in 10 age-matched pairs of 39- to 42-day-old Zucker rats. When obese (fa/fa) were compared to lean (Fa/Fa) rats, relative CO activity was significantly lower (~ 10 percent) in the VMN and parvocellular PVN, but not in the magnocellular PVN. Cell diameters did not differ. To determine if there were corresponding differences in levels or release of hypothalamic monoamines, we compared 7 pairs of 90- to 94-day-old lean (Fa/?) and obese (fa/fa) rats at rest and after 2 h of 9 °C. Tissue punches from frozen PVN, VMN, and preoptic area (the latter being a site of thermosensitive units modulating VMN output) were assayed. In obese vs. lean noncold-exposed rats, we observed lower concentrations of: 5-hydroxyindoleacetic acid (5HIAA; metabolite of serotonin, 5HT) in the VMN; 3-methoxy-4-hydroxyphenylglycol (MHPG; metabolite of norepinephrine, NE) and NE + MHPG (index of total NE) in the preoptic area; and 3,4-dihydroxyphenylacetic acid (DOPAC; metabolite of dopamine, DA) in the PVN. Additionally, in the VMN, cold exposure resulted in: elevated concentrations of MHPG and MHPG + NE in both lean and obese rats; elevated concentrations of 5HT, 5HIAA, and 5HT + 5HIAA in obese rats, with no significant changes in these variables in lean animals; decreased ratio of 5HIAA/5HT in obese rats and increased rats in leans. In the preoptic region, cold exposure led to increased concentrations of MHPG, NE + MHPG, 5HT, and 5HT + 5HIAA in obese but not lean rats. In the PVN, 5HT concentrations were increased in cold-exposed obese but not lean rats. Our data support the hypothesis that neuronal activity in obese rats differs from that of lean rats at rest and during cold exposure and suggest the several monoamine systems play a role in such differences.

AB - Central neural activity was assessed by measuring relative cytochrome oxidase (CO) activity in the ventromedial nucleus (VMN; thermogenesis regulation), the parvocellular paraventricular nucleus (PVN; feeding regulation), and the magnocellular PVN (secretion of vasopressin and oxytocin) in 10 age-matched pairs of 39- to 42-day-old Zucker rats. When obese (fa/fa) were compared to lean (Fa/Fa) rats, relative CO activity was significantly lower (~ 10 percent) in the VMN and parvocellular PVN, but not in the magnocellular PVN. Cell diameters did not differ. To determine if there were corresponding differences in levels or release of hypothalamic monoamines, we compared 7 pairs of 90- to 94-day-old lean (Fa/?) and obese (fa/fa) rats at rest and after 2 h of 9 °C. Tissue punches from frozen PVN, VMN, and preoptic area (the latter being a site of thermosensitive units modulating VMN output) were assayed. In obese vs. lean noncold-exposed rats, we observed lower concentrations of: 5-hydroxyindoleacetic acid (5HIAA; metabolite of serotonin, 5HT) in the VMN; 3-methoxy-4-hydroxyphenylglycol (MHPG; metabolite of norepinephrine, NE) and NE + MHPG (index of total NE) in the preoptic area; and 3,4-dihydroxyphenylacetic acid (DOPAC; metabolite of dopamine, DA) in the PVN. Additionally, in the VMN, cold exposure resulted in: elevated concentrations of MHPG and MHPG + NE in both lean and obese rats; elevated concentrations of 5HT, 5HIAA, and 5HT + 5HIAA in obese rats, with no significant changes in these variables in lean animals; decreased ratio of 5HIAA/5HT in obese rats and increased rats in leans. In the preoptic region, cold exposure led to increased concentrations of MHPG, NE + MHPG, 5HT, and 5HT + 5HIAA in obese but not lean rats. In the PVN, 5HT concentrations were increased in cold-exposed obese but not lean rats. Our data support the hypothesis that neuronal activity in obese rats differs from that of lean rats at rest and during cold exposure and suggest the several monoamine systems play a role in such differences.

KW - brown fat

KW - cytochrome oxidase

KW - hypothalamic monoamines

KW - hypothalamic norepinephrine turnover

KW - hypothalamic serotonin turnover

KW - obesity

KW - paraventricular nucleus

KW - preoptic area

KW - ventromedial nucleus

KW - Zucker rats

UR - http://www.scopus.com/inward/record.url?scp=0025224244&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025224244&partnerID=8YFLogxK

M3 - Article

C2 - 2176650

AN - SCOPUS:0025224244

VL - 14

SP - 879

EP - 891

JO - International Journal of Obesity

JF - International Journal of Obesity

SN - 0307-0565

IS - 10

ER -