Abstract
Current approaches to neural transplantation - adrenal medullary and fetal brain - suffer from limitations in tissue availability, cellular uniformity, and general applicability. The system described here uses temperaturesensitive immortalized clonal neural cells to address these limitations. Cerebellar cells, immortalized with a temperature-sensitive tumor virus, are transformed at 34°C, but differentiate at 38°C. The cells were genetically engineered to express beta-galactosidase, provid-ing a tag to study their fate in the host. The cells were then successfully transplanted to the cerebellum of adult rats.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 205-207 |
| Number of pages | 3 |
| Journal | Annals of Neurology |
| Volume | 27 |
| Issue number | 2 |
| State | Published - Feb 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Neural transplantation using temperature-sensitive immortalized neural cells : A preliminary report. / Bredesen, Dale E.; Hisanaga, Kinya; Sharp, Frank R.
In: Annals of Neurology, Vol. 27, No. 2, 02.1990, p. 205-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neural transplantation using temperature-sensitive immortalized neural cells
T2 - A preliminary report
AU - Bredesen, Dale E.
AU - Hisanaga, Kinya
AU - Sharp, Frank R
PY - 1990/2
Y1 - 1990/2
N2 - Current approaches to neural transplantation - adrenal medullary and fetal brain - suffer from limitations in tissue availability, cellular uniformity, and general applicability. The system described here uses temperaturesensitive immortalized clonal neural cells to address these limitations. Cerebellar cells, immortalized with a temperature-sensitive tumor virus, are transformed at 34°C, but differentiate at 38°C. The cells were genetically engineered to express beta-galactosidase, provid-ing a tag to study their fate in the host. The cells were then successfully transplanted to the cerebellum of adult rats.
AB - Current approaches to neural transplantation - adrenal medullary and fetal brain - suffer from limitations in tissue availability, cellular uniformity, and general applicability. The system described here uses temperaturesensitive immortalized clonal neural cells to address these limitations. Cerebellar cells, immortalized with a temperature-sensitive tumor virus, are transformed at 34°C, but differentiate at 38°C. The cells were genetically engineered to express beta-galactosidase, provid-ing a tag to study their fate in the host. The cells were then successfully transplanted to the cerebellum of adult rats.
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M3 - Article
C2 - 2156481
AN - SCOPUS:0025231691
VL - 27
SP - 205
EP - 207
JO - Annals of Neurology
JF - Annals of Neurology
SN - 0364-5134
IS - 2
ER -