Neural cell adhesion molecule contributes to hemopoiesis-supporting capacity of stromal cell lines

Xiaoli Wang, Hiroko Hisha, Shigeru Taketani, Muneo Inaba, Qiang Li, Wenhao Cui, Changye Song, Tianxue Fan, Yunze Cui, Kequan Guo, Guoxiang Yang, Hongxue Fan, Zhexiong Lian, M. Eric Gershwin, Susumu Ikehara

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


To clarify mechanisms underlying cell-to-cell interactions between hemopoietic stem cells (HSCs) and stromal cells, we established a stromal cell line (FMS/PA6-P) from day-16 fetal bone marrow (BM) adherent cells using an anti-PA6 monoclonal antibody (mAb) specific for BM stromal cells. Importantly, this FMS/PA6-P cell line, showing homogenous fibroblastic morphology, is absent from hematolymphoid and endothelial lineage markers and maintains a high level of expression of PA6 molecule, recognized by the anti-PA6 mAb, for approximately 20 passages. Further, the cell line expressing a high level of PA6 molecule has a better hemopoiesis-supporting capacity in vitro than other stromal cell lines such as PA6 and MS-5. In fact, the PA6 molecule is closely related to the hemopoiesis-supporting capacity of the stromal cells because the proliferation of HSCs was suppressed to a great extent by the anti-PA6 mAb. Affinity chromatography and mass peptide fingerprinting revealed that the protein reacting with the anti-PA6 mAb is neural cell adhesion molecule (NCAM). The frequencies of long-term cobblestone area-forming cells and long-term culture-initiating cells were significantly suppressed by repression of NCAM in the FMS/PA6-P cells using NCAM small interfering RNA. Our Findings clearly indicate that NCAM functions on the maintenance of HSCs.

Original languageEnglish (US)
Pages (from-to)1389-1399
Number of pages11
JournalStem Cells
Issue number9
StatePublished - Oct 2005


  • Bone marrow stromal cells
  • Mouse
  • Neural cell adhesion molecule
  • PA6
  • Pluripotent hemopoietic stem cells

ASJC Scopus subject areas

  • Cell Biology


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