Abstract
Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 79-94 |
| Number of pages | 16 |
| Journal | Progress in Brain Research |
| Volume | 196 |
| DOIs | |
| State | Published - 2012 |
| Externally published | Yes |
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Keywords
- Calcium imaging
- GCaMP3
- Genetically encoded calcium indicators
- Neural activity imaging
- Protein calcium sensors
- Protein engineering
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Neural activity imaging with genetically encoded calcium indicators. / Tian, Lin; Akerboom, Jasper; Schreiter, Eric R.; Looger, Loren L.
In: Progress in Brain Research, Vol. 196, 2012, p. 79-94.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Neural activity imaging with genetically encoded calcium indicators
AU - Tian, Lin
AU - Akerboom, Jasper
AU - Schreiter, Eric R.
AU - Looger, Loren L.
PY - 2012
Y1 - 2012
N2 - Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.
AB - Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.
KW - Calcium imaging
KW - GCaMP3
KW - Genetically encoded calcium indicators
KW - Neural activity imaging
KW - Protein calcium sensors
KW - Protein engineering
UR - http://www.scopus.com/inward/record.url?scp=84857089164&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857089164&partnerID=8YFLogxK
U2 - 10.1016/B978-0-444-59426-6.00005-7
DO - 10.1016/B978-0-444-59426-6.00005-7
M3 - Article
C2 - 22341322
AN - SCOPUS:84857089164
VL - 196
SP - 79
EP - 94
JO - Progress in Brain Research
JF - Progress in Brain Research
SN - 0079-6123
ER -