Neural activity imaging with genetically encoded calcium indicators

Lin Tian, Jasper Akerboom, Eric R. Schreiter, Loren L. Looger

Research output: Contribution to journalArticlepeer-review

42 Scopus citations


Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.

Original languageEnglish (US)
Pages (from-to)79-94
Number of pages16
JournalProgress in Brain Research
StatePublished - 2012
Externally publishedYes


  • Calcium imaging
  • GCaMP3
  • Genetically encoded calcium indicators
  • Neural activity imaging
  • Protein calcium sensors
  • Protein engineering

ASJC Scopus subject areas

  • Neuroscience(all)


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