Neural activity imaging with genetically encoded calcium indicators

Lin Tian; Jasper Akerboom; Eric R. Schreiter; Loren L. Looger

doi:10.1016/B978-0-444-59426-6.00005-7

Neural activity imaging with genetically encoded calcium indicators

Lin Tian, Jasper Akerboom, Eric R. Schreiter, Loren L. Looger

Research output: Contribution to journal › Article

34 Scopus citations

Abstract

Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.

Original language	English (US)
Pages (from-to)	79-94
Number of pages	16
Journal	Progress in Brain Research
Volume	196
DOIs	https://doi.org/10.1016/B978-0-444-59426-6.00005-7
State	Published - 2012
Externally published	Yes

Fingerprint

Keywords

Calcium imaging
GCaMP3
Genetically encoded calcium indicators
Neural activity imaging
Protein calcium sensors
Protein engineering

ASJC Scopus subject areas

Neuroscience(all)

Cite this

Tian, L., Akerboom, J., Schreiter, E. R., & Looger, L. L. (2012). Neural activity imaging with genetically encoded calcium indicators. Progress in Brain Research, 196, 79-94. https://doi.org/10.1016/B978-0-444-59426-6.00005-7

@article{4cefd71ccd7c4954bf0007506671e388,

title = "Neural activity imaging with genetically encoded calcium indicators",

abstract = "Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.",

keywords = "Calcium imaging, GCaMP3, Genetically encoded calcium indicators, Neural activity imaging, Protein calcium sensors, Protein engineering",

author = "Lin Tian and Jasper Akerboom and Schreiter, {Eric R.} and Looger, {Loren L.}",

year = "2012",

doi = "10.1016/B978-0-444-59426-6.00005-7",

language = "English (US)",

volume = "196",

pages = "79--94",

journal = "Progress in Brain Research",

issn = "0079-6123",

publisher = "Elsevier",

}

TY - JOUR

T1 - Neural activity imaging with genetically encoded calcium indicators

AU - Tian, Lin

AU - Akerboom, Jasper

AU - Schreiter, Eric R.

AU - Looger, Loren L.

PY - 2012

Y1 - 2012

N2 - Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.

AB - Genetically encoded calcium indicators (GECIs), together with modern microscopy, allow repeated activity measurement, in real time and with cellular resolution, of defined cellular populations. Recent efforts in protein engineering have yielded several high-quality GECIs that facilitate new applications in neuroscience. Here, we summarize recent progress in GECI design, optimization, and characterization, and provide guidelines for selecting the appropriate GECI for a given biological application. We focus on the unique challenges associated with imaging in behaving animals.

KW - Calcium imaging

KW - GCaMP3

KW - Genetically encoded calcium indicators

KW - Neural activity imaging

KW - Protein calcium sensors

KW - Protein engineering

UR - http://www.scopus.com/inward/record.url?scp=84857089164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857089164&partnerID=8YFLogxK

U2 - 10.1016/B978-0-444-59426-6.00005-7

DO - 10.1016/B978-0-444-59426-6.00005-7

M3 - Article

C2 - 22341322

AN - SCOPUS:84857089164

VL - 196

SP - 79

EP - 94

JO - Progress in Brain Research

JF - Progress in Brain Research

SN - 0079-6123

ER -

Access to Document

10.1016/B978-0-444-59426-6.00005-7