Nested polymerase chain reaction for detection of Ehrlichia risticii genomic DNA in infected horses

Jeffrey E. Barlough, Yasuko Rikihisa, John E Madigan

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

A nested polymerase chain reaction was developed for amplifying a 529-bp segment of the 16S ribosomal RNA gene of Ehrlichia risticii from equine buffy coat cells. Confirmation of identity of the amplified bands was accomplished by Southern hybridization and DNA sequencing. The study indicated a detection limit of > 10 copies of the target gene, and specificity for E. risticii as based on a panel of test rickettsiae. Ticks (Ixodes pacificus) collected in an area of northern California enzootic for equine monocytic ehrlichiosis were found to be negative for E. risticii DNA.

Original languageEnglish (US)
Pages (from-to)367-373
Number of pages7
JournalVeterinary Parasitology
Volume68
Issue number4
DOIs
StatePublished - Mar 1997

Keywords

  • diagnosis-Rickettsia
  • DNA sequence analysis
  • Ehrlichia risticii
  • equine monocytic ehrlichiosis
  • horse
  • Ixodes pacificus
  • polymerase chain reaction
  • potomac horse fever
  • Southern hybridization test

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

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