Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate tetrahymena thermophila

Erica Zweifel, Joshua Smith, Daniel Romero, Thomas H. Giddings, Mark Winey, Jerry Honts, Jeff Dahlseid, Brent Schneider, Eric S. Cole

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We describe a novel pair of nested genes, CDA12 and CDA13, from Tetrahymena thermophila. Both are implicated in membrane trafficking associated with cell division and conjugation. Green fluorescent protein localization reveals Cda12p decoration of diverse membrane-bound compartments, including mobile, subcortical tubulovesicular compartments; perinuclear vesicles; and candidates for recycling endosomes. Cda13p decorates intracellular foci located adjacent to cortically aligned mitochondria and their neighboring Golgi networks. The expression of antisense CDA12 RNA in transformants produces defects in cytokinesis, macronuclear segregation, and the processing of pinosomes to downstream compartments. Antisense CDA13 RNA expression produces a conjugation phenotype, resulting in the failure of mating pairs to separate, as well as failures in postconjugation cytokinesis and macronuclear fission. This study offers insight into the membrane trafficking events linking endosóme and Golgi network activities, cytokinesis, and karyokinesis and the unique membrane-remodeling events that accompany conjugation in the ciliate T. thermophila. We also highlight an unusual aspect of genome organization in Tetrahymena, namely, the existence of nested, antisense genes.

Original languageEnglish (US)
Pages (from-to)899-912
Number of pages14
JournalEukaryotic Cell
Volume8
Issue number6
DOIs
StatePublished - Jun 1 2009
Externally publishedYes

Fingerprint

Nested Genes
Tetrahymena thermophila
Cytokinesis
Membrane Proteins
Antisense RNA
Membranes
Cell Nucleus Division
Tetrahymena
Endosomes
Green Fluorescent Proteins
Cell Division
Mitochondria
Genome
Phenotype

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate tetrahymena thermophila. / Zweifel, Erica; Smith, Joshua; Romero, Daniel; Giddings, Thomas H.; Winey, Mark; Honts, Jerry; Dahlseid, Jeff; Schneider, Brent; Cole, Eric S.

In: Eukaryotic Cell, Vol. 8, No. 6, 01.06.2009, p. 899-912.

Research output: Contribution to journalArticle

Zweifel, E, Smith, J, Romero, D, Giddings, TH, Winey, M, Honts, J, Dahlseid, J, Schneider, B & Cole, ES 2009, 'Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate tetrahymena thermophila', Eukaryotic Cell, vol. 8, no. 6, pp. 899-912. https://doi.org/10.1128/EC.00342-08
Zweifel, Erica ; Smith, Joshua ; Romero, Daniel ; Giddings, Thomas H. ; Winey, Mark ; Honts, Jerry ; Dahlseid, Jeff ; Schneider, Brent ; Cole, Eric S. / Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate tetrahymena thermophila. In: Eukaryotic Cell. 2009 ; Vol. 8, No. 6. pp. 899-912.
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