Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells

Nutan Sharma, Gabriella D'Arcangelo, Amy Kleinklaus, Simon Halegoua, James Trimmer

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifier K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of Mr = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 K+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.

Original languageEnglish (US)
Pages (from-to)1835-1843
Number of pages9
JournalJournal of Cell Biology
Volume123
Issue number6 PART 2
StatePublished - Dec 1993
Externally publishedYes

Fingerprint

PC12 Cells
Nerve Growth Factor
Peptides
Staining and Labeling
Cell Membrane
Growth Cones
Ribonucleases
Population
Anti-Idiotypic Antibodies
Messenger RNA
Membranes
Brain
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Sharma, N., D'Arcangelo, G., Kleinklaus, A., Halegoua, S., & Trimmer, J. (1993). Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. Journal of Cell Biology, 123(6 PART 2), 1835-1843.

Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. / Sharma, Nutan; D'Arcangelo, Gabriella; Kleinklaus, Amy; Halegoua, Simon; Trimmer, James.

In: Journal of Cell Biology, Vol. 123, No. 6 PART 2, 12.1993, p. 1835-1843.

Research output: Contribution to journalArticle

Sharma, N, D'Arcangelo, G, Kleinklaus, A, Halegoua, S & Trimmer, J 1993, 'Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells', Journal of Cell Biology, vol. 123, no. 6 PART 2, pp. 1835-1843.
Sharma N, D'Arcangelo G, Kleinklaus A, Halegoua S, Trimmer J. Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. Journal of Cell Biology. 1993 Dec;123(6 PART 2):1835-1843.
Sharma, Nutan ; D'Arcangelo, Gabriella ; Kleinklaus, Amy ; Halegoua, Simon ; Trimmer, James. / Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells. In: Journal of Cell Biology. 1993 ; Vol. 123, No. 6 PART 2. pp. 1835-1843.
@article{966b9b15168c4b48b5ad0ef84af8b2df,
title = "Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells",
abstract = "We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifier K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of Mr = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 K+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.",
author = "Nutan Sharma and Gabriella D'Arcangelo and Amy Kleinklaus and Simon Halegoua and James Trimmer",
year = "1993",
month = "12",
language = "English (US)",
volume = "123",
pages = "1835--1843",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "6 PART 2",

}

TY - JOUR

T1 - Nerve growth factor regulates the abundance and distribution of K+ channels in PC12 cells

AU - Sharma, Nutan

AU - D'Arcangelo, Gabriella

AU - Kleinklaus, Amy

AU - Halegoua, Simon

AU - Trimmer, James

PY - 1993/12

Y1 - 1993/12

N2 - We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifier K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of Mr = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 K+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.

AB - We examined the effect of nerve growth factor (NGF) treatment on expression of a neuronal delayed rectifier K+ channel subtype, Kv2.1 (drk1), in PC12 cells. Anti-Kv2.1 antibodies recognized a single polypeptide population of Mr = 132 kD in PC12 cell membranes, distinct from the more heterogeneous population found in adult rat brain. In response to NGF treatment, levels of Kv2.1 polypeptide in PC12 membranes increased fourfold. This increase in polypeptide levels could be seen within 12 h, and elevated levels were maintained for at least 6 d of continuous NGF treatment. RNase protection assays indicate that this increase in Kv2.1 protein occurs without an increase in steady state levels of Kv2.1 mRNA following NGF treatment. Immunofluorescent localization of the Kv2.1 polypeptide revealed plasma membrane-associated staining of cell bodies in both untreated and NGF-treated PC12 cells. In undifferentiated cells, intense staining is seen at sites of cell-cell and cell-substratum contact. In differentiated cells the most intense Kv2.1 staining is observed in neuritic growth cones. These studies show that in PC12 cells both the abundance and distribution of the Kv2.1 K+ channel are regulated by NGF, and suggest that PC12 cells provide a model for the selective expression of Kv2.1 in neuritic endings.

UR - http://www.scopus.com/inward/record.url?scp=0027714923&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027714923&partnerID=8YFLogxK

M3 - Article

C2 - 8276901

AN - SCOPUS:0027714923

VL - 123

SP - 1835

EP - 1843

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 6 PART 2

ER -