Neoplastic transformation of prostatic and urogenital epithelium by the polyoma virus middle T gene

Arash Tehranian, David W. Morris, Byung Hee Min, Dana J. Bird, Robert Cardiff, Peter A Barry

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Male transgenic mice expressing the polyomavirus middle T (PyV-MT) gene exhibited growth and developmental abnormalities in prostatic and other urogenital epithelium. Expression of PyV-MT was directed to these tissues by a novel, androgen-inducible expression vector based on the rat C3(1) gene. Epithelial growth disturbances (hyperplasia, dysplasia, and invasive carcinoma) were observed in the ventral and dorsal prostate, coagulating gland, epididymis, and vas deferens. The abnormalities were characterized by histological disorganization, nuclear pleomorphism, increased mitoses, and ab normal DNA content. Transgene transcription was detected in affected tissues, indicating that the C3(1)-based vector targeted androgen-sensitive urogenital tissues, especially the prostate. These results demonstrated that expression of a gene, the protein of which is known to interact with cellular proteins involved in signal transduction, dramatically disrupted urogenital growth and development.

Original languageEnglish (US)
Pages (from-to)1177-1191
Number of pages15
JournalAmerican Journal of Pathology
Volume149
Issue number4
StatePublished - Oct 1996

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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