Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress

Johan D. Söderholm, Derrick A. Yates, Melanie Gareau, Ping Chang Yang, Glenda MacQueen, Mary H. Perdue

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

Intestinal dysfunction is related to stress and early life events, but the mechanisms are largely unknown. Our aim was to determine whether early trauma predisposes adult rats to intestinal mucosal dysfunction in response to stress. Neonatal Sprague-Dawley rats were individually separated from their mothers for 3 h/day at 4-21 days of age. Between days 80 and 90, separated and control rats were subjected to mild acute stress (30-min water avoidance) or sham stress. Mucosal barrier function and ion transport were assessed in colonic tissues mounted in Ussing chambers. Mild stress increased short-circuit current, conductance, and transepithelial transport of macromolecules in separated rats, while having minimal effects in controls. Pretreatment of the separated rats with a corticotropin-releasing hormone (CRH) antagonist, the peptide α-helical CRH(9-41) injected intraperitoneally 20 min before stress, abolished the stress-induced mucosal changes. Our results indicate that neonatal trauma can induce phenotypic changes in adulthood, including enhanced vulnerability of the gut mucosa to stress via mechanisms involving peripherally located CRH receptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume283
Issue number6 46-6
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

Mothers
Corticotropin-Releasing Hormone Receptors
Hormone Antagonists
Corticotropin-Releasing Hormone
Ion Transport
Wounds and Injuries
Psychological Stress
Sprague Dawley Rats
Mucous Membrane
Peptides
Water

Keywords

  • Behavior
  • Corticotropin-releasing hormone
  • Electron microscopy
  • Intestinal permeability
  • Ion transport

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology

Cite this

Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress. / Söderholm, Johan D.; Yates, Derrick A.; Gareau, Melanie; Yang, Ping Chang; MacQueen, Glenda; Perdue, Mary H.

In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 283, No. 6 46-6, 01.12.2002.

Research output: Contribution to journalArticle

Söderholm, Johan D. ; Yates, Derrick A. ; Gareau, Melanie ; Yang, Ping Chang ; MacQueen, Glenda ; Perdue, Mary H. / Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress. In: American Journal of Physiology - Gastrointestinal and Liver Physiology. 2002 ; Vol. 283, No. 6 46-6.
@article{adaa2efecee1499abdc60e8dfc2189a6,
title = "Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress",
abstract = "Intestinal dysfunction is related to stress and early life events, but the mechanisms are largely unknown. Our aim was to determine whether early trauma predisposes adult rats to intestinal mucosal dysfunction in response to stress. Neonatal Sprague-Dawley rats were individually separated from their mothers for 3 h/day at 4-21 days of age. Between days 80 and 90, separated and control rats were subjected to mild acute stress (30-min water avoidance) or sham stress. Mucosal barrier function and ion transport were assessed in colonic tissues mounted in Ussing chambers. Mild stress increased short-circuit current, conductance, and transepithelial transport of macromolecules in separated rats, while having minimal effects in controls. Pretreatment of the separated rats with a corticotropin-releasing hormone (CRH) antagonist, the peptide α-helical CRH(9-41) injected intraperitoneally 20 min before stress, abolished the stress-induced mucosal changes. Our results indicate that neonatal trauma can induce phenotypic changes in adulthood, including enhanced vulnerability of the gut mucosa to stress via mechanisms involving peripherally located CRH receptors.",
keywords = "Behavior, Corticotropin-releasing hormone, Electron microscopy, Intestinal permeability, Ion transport",
author = "S{\"o}derholm, {Johan D.} and Yates, {Derrick A.} and Melanie Gareau and Yang, {Ping Chang} and Glenda MacQueen and Perdue, {Mary H.}",
year = "2002",
month = "12",
day = "1",
language = "English (US)",
volume = "283",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "6 46-6",

}

TY - JOUR

T1 - Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress

AU - Söderholm, Johan D.

AU - Yates, Derrick A.

AU - Gareau, Melanie

AU - Yang, Ping Chang

AU - MacQueen, Glenda

AU - Perdue, Mary H.

PY - 2002/12/1

Y1 - 2002/12/1

N2 - Intestinal dysfunction is related to stress and early life events, but the mechanisms are largely unknown. Our aim was to determine whether early trauma predisposes adult rats to intestinal mucosal dysfunction in response to stress. Neonatal Sprague-Dawley rats were individually separated from their mothers for 3 h/day at 4-21 days of age. Between days 80 and 90, separated and control rats were subjected to mild acute stress (30-min water avoidance) or sham stress. Mucosal barrier function and ion transport were assessed in colonic tissues mounted in Ussing chambers. Mild stress increased short-circuit current, conductance, and transepithelial transport of macromolecules in separated rats, while having minimal effects in controls. Pretreatment of the separated rats with a corticotropin-releasing hormone (CRH) antagonist, the peptide α-helical CRH(9-41) injected intraperitoneally 20 min before stress, abolished the stress-induced mucosal changes. Our results indicate that neonatal trauma can induce phenotypic changes in adulthood, including enhanced vulnerability of the gut mucosa to stress via mechanisms involving peripherally located CRH receptors.

AB - Intestinal dysfunction is related to stress and early life events, but the mechanisms are largely unknown. Our aim was to determine whether early trauma predisposes adult rats to intestinal mucosal dysfunction in response to stress. Neonatal Sprague-Dawley rats were individually separated from their mothers for 3 h/day at 4-21 days of age. Between days 80 and 90, separated and control rats were subjected to mild acute stress (30-min water avoidance) or sham stress. Mucosal barrier function and ion transport were assessed in colonic tissues mounted in Ussing chambers. Mild stress increased short-circuit current, conductance, and transepithelial transport of macromolecules in separated rats, while having minimal effects in controls. Pretreatment of the separated rats with a corticotropin-releasing hormone (CRH) antagonist, the peptide α-helical CRH(9-41) injected intraperitoneally 20 min before stress, abolished the stress-induced mucosal changes. Our results indicate that neonatal trauma can induce phenotypic changes in adulthood, including enhanced vulnerability of the gut mucosa to stress via mechanisms involving peripherally located CRH receptors.

KW - Behavior

KW - Corticotropin-releasing hormone

KW - Electron microscopy

KW - Intestinal permeability

KW - Ion transport

UR - http://www.scopus.com/inward/record.url?scp=0036890453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036890453&partnerID=8YFLogxK

M3 - Article

C2 - 12388189

AN - SCOPUS:0036890453

VL - 283

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 6 46-6

ER -