Neonatal maternal separation predisposes adult rats to colonic barrier dysfunction in response to mild stress

Johan D. Söderholm, Derrick A. Yates, Melanie Gareau, Ping Chang Yang, Glenda MacQueen, Mary H. Perdue

Research output: Contribution to journalArticlepeer-review

202 Scopus citations


Intestinal dysfunction is related to stress and early life events, but the mechanisms are largely unknown. Our aim was to determine whether early trauma predisposes adult rats to intestinal mucosal dysfunction in response to stress. Neonatal Sprague-Dawley rats were individually separated from their mothers for 3 h/day at 4-21 days of age. Between days 80 and 90, separated and control rats were subjected to mild acute stress (30-min water avoidance) or sham stress. Mucosal barrier function and ion transport were assessed in colonic tissues mounted in Ussing chambers. Mild stress increased short-circuit current, conductance, and transepithelial transport of macromolecules in separated rats, while having minimal effects in controls. Pretreatment of the separated rats with a corticotropin-releasing hormone (CRH) antagonist, the peptide α-helical CRH(9-41) injected intraperitoneally 20 min before stress, abolished the stress-induced mucosal changes. Our results indicate that neonatal trauma can induce phenotypic changes in adulthood, including enhanced vulnerability of the gut mucosa to stress via mechanisms involving peripherally located CRH receptors.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Issue number6 46-6
StatePublished - Dec 1 2002
Externally publishedYes


  • Behavior
  • Corticotropin-releasing hormone
  • Electron microscopy
  • Intestinal permeability
  • Ion transport

ASJC Scopus subject areas

  • Gastroenterology
  • Physiology


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