Neonatal aerosol exposure to Bermuda grass allergen prevents subsequent induction of experimental allergic feline asthma: Evidence for establishing early immunologic tolerance

Meera C Heller, T. M. Lee-Fowler, H. Liu, L. A. Cohn, C. R. Reinero

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Allergic asthma is increasing in industrialized countries, especially in children. Rodent and human studies suggest an opportunity to "prevent" asthma in the perinatal period. The aims of this study were to create a more "natural" model of feline asthma by exposing offspring of asthmatic queens to Bermuda grass allergen (BGA) by inhalation only, and to investigate maternal-fetal-infant interactions in the development of asthma. Kittens from asthmatic queens were divided into four groups: maternal exposure to aerosolized BGA during the third trimester, neonatal exposure to aerosolized BGA in the first three months of life, both maternal and neonatal exposure, or saline control. Kittens failing to achieve an asthmatic phenotype based on bronchoalveolar lavage fluid (BALF) analysis by 6 months underwent traditional sensitization: adjuvanted allergen injection, intranasal allergen, and aerosol challenges. BALF was collected at 3, 4 and 6 months, and after sensitization at 8 months, and analyzed for eosinophil counts and BGA-specific IgG and IgA. Intradermal testing (IDT) was performed at 6 and 7 months. At six months none of the kittens had airway eosinophilia, BGA-specific IgG or IgA, and were non-responsive to IDT. After sensitization, kittens receiving neonatal aerosolization failed to develop airway eosinophilia as seen in the controls. Kittens exposed to BGA aerosols, either in-utero or neonatally, continued to lack IDT response. Chronic exposure to BGA aerosols failed to induce asthma in kittens, and instead tolerized the kittens to BGA. This is the first evidence that neonatal intervention could potentially "prevent" allergic asthma in cats.

Original languageEnglish (US)
Pages (from-to)20-25
Number of pages6
JournalVeterinary Immunology and Immunopathology
Volume160
Issue number1-2
DOIs
StatePublished - Jul 15 2014
Externally publishedYes

Fingerprint

Cynodon
Felidae
asthma
Cynodon dactylon
aerosols
allergens
Aerosols
Allergens
kittens
Asthma
cats
Maternal Exposure
eosinophilia
Bronchoalveolar Lavage Fluid
Eosinophilia
Immunoglobulin A
Immunoglobulin G
perinatal period
testing
Third Pregnancy Trimester

Keywords

  • Allergic asthma
  • Bermuda grass allergen
  • Feline asthma
  • Neonatal immunity
  • Tolerance

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)
  • Medicine(all)

Cite this

Neonatal aerosol exposure to Bermuda grass allergen prevents subsequent induction of experimental allergic feline asthma : Evidence for establishing early immunologic tolerance. / Heller, Meera C; Lee-Fowler, T. M.; Liu, H.; Cohn, L. A.; Reinero, C. R.

In: Veterinary Immunology and Immunopathology, Vol. 160, No. 1-2, 15.07.2014, p. 20-25.

Research output: Contribution to journalArticle

@article{d84d030bf0c94044b850ce4f06f880f9,
title = "Neonatal aerosol exposure to Bermuda grass allergen prevents subsequent induction of experimental allergic feline asthma: Evidence for establishing early immunologic tolerance",
abstract = "Allergic asthma is increasing in industrialized countries, especially in children. Rodent and human studies suggest an opportunity to {"}prevent{"} asthma in the perinatal period. The aims of this study were to create a more {"}natural{"} model of feline asthma by exposing offspring of asthmatic queens to Bermuda grass allergen (BGA) by inhalation only, and to investigate maternal-fetal-infant interactions in the development of asthma. Kittens from asthmatic queens were divided into four groups: maternal exposure to aerosolized BGA during the third trimester, neonatal exposure to aerosolized BGA in the first three months of life, both maternal and neonatal exposure, or saline control. Kittens failing to achieve an asthmatic phenotype based on bronchoalveolar lavage fluid (BALF) analysis by 6 months underwent traditional sensitization: adjuvanted allergen injection, intranasal allergen, and aerosol challenges. BALF was collected at 3, 4 and 6 months, and after sensitization at 8 months, and analyzed for eosinophil counts and BGA-specific IgG and IgA. Intradermal testing (IDT) was performed at 6 and 7 months. At six months none of the kittens had airway eosinophilia, BGA-specific IgG or IgA, and were non-responsive to IDT. After sensitization, kittens receiving neonatal aerosolization failed to develop airway eosinophilia as seen in the controls. Kittens exposed to BGA aerosols, either in-utero or neonatally, continued to lack IDT response. Chronic exposure to BGA aerosols failed to induce asthma in kittens, and instead tolerized the kittens to BGA. This is the first evidence that neonatal intervention could potentially {"}prevent{"} allergic asthma in cats.",
keywords = "Allergic asthma, Bermuda grass allergen, Feline asthma, Neonatal immunity, Tolerance",
author = "Heller, {Meera C} and Lee-Fowler, {T. M.} and H. Liu and Cohn, {L. A.} and Reinero, {C. R.}",
year = "2014",
month = "7",
day = "15",
doi = "10.1016/j.vetimm.2014.03.006",
language = "English (US)",
volume = "160",
pages = "20--25",
journal = "Veterinary Immunology and Immunopathology",
issn = "0165-2427",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Neonatal aerosol exposure to Bermuda grass allergen prevents subsequent induction of experimental allergic feline asthma

T2 - Evidence for establishing early immunologic tolerance

AU - Heller, Meera C

AU - Lee-Fowler, T. M.

AU - Liu, H.

AU - Cohn, L. A.

AU - Reinero, C. R.

PY - 2014/7/15

Y1 - 2014/7/15

N2 - Allergic asthma is increasing in industrialized countries, especially in children. Rodent and human studies suggest an opportunity to "prevent" asthma in the perinatal period. The aims of this study were to create a more "natural" model of feline asthma by exposing offspring of asthmatic queens to Bermuda grass allergen (BGA) by inhalation only, and to investigate maternal-fetal-infant interactions in the development of asthma. Kittens from asthmatic queens were divided into four groups: maternal exposure to aerosolized BGA during the third trimester, neonatal exposure to aerosolized BGA in the first three months of life, both maternal and neonatal exposure, or saline control. Kittens failing to achieve an asthmatic phenotype based on bronchoalveolar lavage fluid (BALF) analysis by 6 months underwent traditional sensitization: adjuvanted allergen injection, intranasal allergen, and aerosol challenges. BALF was collected at 3, 4 and 6 months, and after sensitization at 8 months, and analyzed for eosinophil counts and BGA-specific IgG and IgA. Intradermal testing (IDT) was performed at 6 and 7 months. At six months none of the kittens had airway eosinophilia, BGA-specific IgG or IgA, and were non-responsive to IDT. After sensitization, kittens receiving neonatal aerosolization failed to develop airway eosinophilia as seen in the controls. Kittens exposed to BGA aerosols, either in-utero or neonatally, continued to lack IDT response. Chronic exposure to BGA aerosols failed to induce asthma in kittens, and instead tolerized the kittens to BGA. This is the first evidence that neonatal intervention could potentially "prevent" allergic asthma in cats.

AB - Allergic asthma is increasing in industrialized countries, especially in children. Rodent and human studies suggest an opportunity to "prevent" asthma in the perinatal period. The aims of this study were to create a more "natural" model of feline asthma by exposing offspring of asthmatic queens to Bermuda grass allergen (BGA) by inhalation only, and to investigate maternal-fetal-infant interactions in the development of asthma. Kittens from asthmatic queens were divided into four groups: maternal exposure to aerosolized BGA during the third trimester, neonatal exposure to aerosolized BGA in the first three months of life, both maternal and neonatal exposure, or saline control. Kittens failing to achieve an asthmatic phenotype based on bronchoalveolar lavage fluid (BALF) analysis by 6 months underwent traditional sensitization: adjuvanted allergen injection, intranasal allergen, and aerosol challenges. BALF was collected at 3, 4 and 6 months, and after sensitization at 8 months, and analyzed for eosinophil counts and BGA-specific IgG and IgA. Intradermal testing (IDT) was performed at 6 and 7 months. At six months none of the kittens had airway eosinophilia, BGA-specific IgG or IgA, and were non-responsive to IDT. After sensitization, kittens receiving neonatal aerosolization failed to develop airway eosinophilia as seen in the controls. Kittens exposed to BGA aerosols, either in-utero or neonatally, continued to lack IDT response. Chronic exposure to BGA aerosols failed to induce asthma in kittens, and instead tolerized the kittens to BGA. This is the first evidence that neonatal intervention could potentially "prevent" allergic asthma in cats.

KW - Allergic asthma

KW - Bermuda grass allergen

KW - Feline asthma

KW - Neonatal immunity

KW - Tolerance

UR - http://www.scopus.com/inward/record.url?scp=84901605450&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901605450&partnerID=8YFLogxK

U2 - 10.1016/j.vetimm.2014.03.006

DO - 10.1016/j.vetimm.2014.03.006

M3 - Article

C2 - 24704287

AN - SCOPUS:84901605450

VL - 160

SP - 20

EP - 25

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 1-2

ER -