Near-infrared optical imaging of ovarian cancer xenografts with novel α3-integrin binding peptide "OA02"

Olulanu H. Aina, Jan Marik, Regina F Gandour-Edwards, Kit Lam

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Through screening of random one-bead one-compound (OBOC) libraries, we previously identified cyclic peptides with the cDGXGXXc motif that bind to α3 integrin subunit on ovarian adenocarcinoma cell lines ES-2, SKOV-3, and CaOV-3. We subsequently synthesized two secondary libraries based on this motif and identified new peptides that bound with a higher affinity to these cell lines. One of the peptides identified from the 20% "down-substituted" focused library was the cdGHCit-GPQc ("OA02") peptide. The goal of this study was to determine whether this peptide labeled with near-infrared probes could be detected after intravenous injection in ovarian tumor-bearing mice and if it would selectively localize in the tumor. Three different forms of this peptide were synthesized, "OA02"-biotin (noncovalently linked to streptavidin-Cy5.5); "OA02"-Cy5.5 and "OA02"-AlexaFluo 680. Using a KODAK IS2000MM image station, these peptide probes were used at the near-infrared (NIR) spectra to image nude mice bearing ES-2 (α3 integrin positive) and Raji (α3 integrin negative) xenografts. The peptide probe displayed highly specific tumor uptake within 15 min, which lasted for 70 min for "OA02"Cy5.5 and "OA02"-AlexaFluo 680 and for 24 hours for "OA02"-biotin-streptavidin-Cy5.5. Some kidney and bladder signal were noted. Prior injection with anti-α3 monoclonal antibody blocked the binding of this peptide to the ES-2 tumors.

Original languageEnglish (US)
Pages (from-to)439-447
Number of pages9
JournalMolecular Imaging
Volume4
Issue number4
StatePublished - Oct 2005

Fingerprint

Optical Imaging
Heterografts
Integrins
Ovarian Neoplasms
Peptides
Infrared radiation
Imaging techniques
Tumors
Bearings (structural)
Libraries
Streptavidin
Biotin
Neoplasms
Cells
Cell Line
Cyclic Peptides
Monoclonal antibodies
Nude Mice
Intravenous Injections
Urinary Bladder

Keywords

  • Combinatorial chemistry
  • Integrin
  • Optical imaging
  • Ovarian cancer
  • Peptides

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Near-infrared optical imaging of ovarian cancer xenografts with novel α3-integrin binding peptide "OA02". / Aina, Olulanu H.; Marik, Jan; Gandour-Edwards, Regina F; Lam, Kit.

In: Molecular Imaging, Vol. 4, No. 4, 10.2005, p. 439-447.

Research output: Contribution to journalArticle

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