Abstract
Near infrared spectroscopy was used to quantify blood content and oxygenation dynamics in abdominal organs and skeletal muscle of 18 anesthetized rabbits during hypoxic hypoxia. Liver, kidney and hindlimb muscle were exposed surgically. Laser diode pulses (775, 8 10, 862 and 904 nm) transmitted across the tissues were detected by means of a photomultiplier. The amount and redox level of tissue hemoglobin were estimated from the near infrared signals and monitored during 5 mm long hypoxic challenges (10% and 6% FiO2) and subsequent recovery periods. In the kidney, exposure to 10% FiO2 resulted in rapid and symmetrical changes in oxygenated and reduced hemoglobin with 50% of the variations occurring within 1 mm and a plateau after 3 min. Total hemoglobin did not change and hemoglobin oxygenation returned to baseline within 1 mm of hypoxia cessation. Exposure to 6% FiO2 doubled the decrease in oxygenated hemoglobin and induced a sustained vasoconstriction which decreased total hemoglobin content 2 min after initiation of hypoxia. Comparable patterns were observed in the liver and skeletal muscle with the following exceptions: Local vasoconstriction was generally not observed at 6% FiO2, return to baseline oxygen availability was much slower in skeletal muscle than in the other organs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 157-165 |
| Number of pages | 9 |
| Journal | Proceedings of SPIE - The International Society for Optical Engineering |
| Volume | 2135 |
| DOIs | |
| State | Published - May 19 1994 |
| Event | Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases 1994 - Los Angeles, United States Duration: Jan 23 1994 → Jan 29 1994 |
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ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Computer Science Applications
- Applied Mathematics
- Electrical and Electronic Engineering
Cite this
Near infrared monitoring of perfusion and oxygen availability in abdominal organs and skeletal muscle during hypoxia. / Maarek, Jean Michel; Vari, Sandor; Marcu, Laura; Papaioanou, Thanassis; Pergadia, Vani; Snyder, Wendy; Grundfest, Warren.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 2135, 19.05.1994, p. 157-165.Research output: Contribution to journal › Conference article
}
TY - JOUR
T1 - Near infrared monitoring of perfusion and oxygen availability in abdominal organs and skeletal muscle during hypoxia
AU - Maarek, Jean Michel
AU - Vari, Sandor
AU - Marcu, Laura
AU - Papaioanou, Thanassis
AU - Pergadia, Vani
AU - Snyder, Wendy
AU - Grundfest, Warren
PY - 1994/5/19
Y1 - 1994/5/19
N2 - Near infrared spectroscopy was used to quantify blood content and oxygenation dynamics in abdominal organs and skeletal muscle of 18 anesthetized rabbits during hypoxic hypoxia. Liver, kidney and hindlimb muscle were exposed surgically. Laser diode pulses (775, 8 10, 862 and 904 nm) transmitted across the tissues were detected by means of a photomultiplier. The amount and redox level of tissue hemoglobin were estimated from the near infrared signals and monitored during 5 mm long hypoxic challenges (10% and 6% FiO2) and subsequent recovery periods. In the kidney, exposure to 10% FiO2 resulted in rapid and symmetrical changes in oxygenated and reduced hemoglobin with 50% of the variations occurring within 1 mm and a plateau after 3 min. Total hemoglobin did not change and hemoglobin oxygenation returned to baseline within 1 mm of hypoxia cessation. Exposure to 6% FiO2 doubled the decrease in oxygenated hemoglobin and induced a sustained vasoconstriction which decreased total hemoglobin content 2 min after initiation of hypoxia. Comparable patterns were observed in the liver and skeletal muscle with the following exceptions: Local vasoconstriction was generally not observed at 6% FiO2, return to baseline oxygen availability was much slower in skeletal muscle than in the other organs.
AB - Near infrared spectroscopy was used to quantify blood content and oxygenation dynamics in abdominal organs and skeletal muscle of 18 anesthetized rabbits during hypoxic hypoxia. Liver, kidney and hindlimb muscle were exposed surgically. Laser diode pulses (775, 8 10, 862 and 904 nm) transmitted across the tissues were detected by means of a photomultiplier. The amount and redox level of tissue hemoglobin were estimated from the near infrared signals and monitored during 5 mm long hypoxic challenges (10% and 6% FiO2) and subsequent recovery periods. In the kidney, exposure to 10% FiO2 resulted in rapid and symmetrical changes in oxygenated and reduced hemoglobin with 50% of the variations occurring within 1 mm and a plateau after 3 min. Total hemoglobin did not change and hemoglobin oxygenation returned to baseline within 1 mm of hypoxia cessation. Exposure to 6% FiO2 doubled the decrease in oxygenated hemoglobin and induced a sustained vasoconstriction which decreased total hemoglobin content 2 min after initiation of hypoxia. Comparable patterns were observed in the liver and skeletal muscle with the following exceptions: Local vasoconstriction was generally not observed at 6% FiO2, return to baseline oxygen availability was much slower in skeletal muscle than in the other organs.
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U2 - 10.1117/12.175989
DO - 10.1117/12.175989
M3 - Conference article
AN - SCOPUS:85029390742
VL - 2135
SP - 157
EP - 165
JO - Proceedings of SPIE - The International Society for Optical Engineering
JF - Proceedings of SPIE - The International Society for Optical Engineering
SN - 0277-786X
ER -