Near-Infrared Fluorescent Immunoassays: A Novel Approach to Environmental Analysis

A. R. Swamy, M. I. Danesvar, L. Evans, L. Strekowski, N. Narayanan, Ferenc Szurdoki, I. Wengatz, Bruce D. Hammock, G. Patonay

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

The near-infrared (NIR) region of spectrum provides many advantages over conventional UV-visible region and is particularly useful when background interference is the primary concern. Many NIR fluorophores have high molar absorptivities and fluorescence quantum yields. The use of laser diodes for excitation and photodiodes for detection makes the NIR fluorophores an ideal choice as tracers for various applications. NIR fluorescence immunoassays (NIRFIAs) for disease-related antigens have been developed and optimized in our laboratory during the past few years. NIRFIA can be used in both sandwich and competitive formats. We have successfully adapted the NIRFIA as both a microtiter plate assay and a fiber optic probe. This paper is focussed on the environmental analytical applications of this novel technique. Physical properties of ideal NIR labels and engineering problems of devising remote sensors are discussed.

Original languageEnglish (US)
Pages (from-to)146-161
Number of pages16
JournalACS Symposium Series
Volume657
StatePublished - 1996

ASJC Scopus subject areas

  • Chemistry(all)

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