Near-infrared autofluorescence imaging for detection of cancer

Stavros G. Demos, Regina F Gandour-Edwards, Rajen Ramsamooj, Ralph W deVere White

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation. A set of images for each tissue sample was recorded that consisted of two autofluorescence images obtained under 532- and 632.8-nm excitation and lightscattering images obtained under linearly polarized illumination at 700, 850, and 1000 nm. These images were compared with the histopathology of the tissue sample. The experimental results indicated that for various tissue types, the intensity of the autofluorescence integrated over the 700 to 1000-nm spectral region was considerably different in cancer tissues than in that of the contiguous nonneoplastic tissues. This difference provided the basis for the detection of cancer and delineation of the tumor margins. Variations on the relative intensity were observed among different tissue types and excitation wavelengths.

Original languageEnglish (US)
Pages (from-to)587-592
Number of pages6
JournalJournal of Biomedical Optics
Volume9
Issue number3
DOIs
StatePublished - May 2004

Fingerprint

Optical Imaging
Infrared imaging
cancer
Tissue
Neoplasms
excitation
Wavelength
Laser excitation
Elastic scattering
delineation
Lighting
wavelengths
Light scattering
Tumors
margins
elastic scattering
Lasers
light scattering
tumors
illumination

Keywords

  • Autofluorescence
  • Cancer
  • Near-infrared

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Near-infrared autofluorescence imaging for detection of cancer. / Demos, Stavros G.; Gandour-Edwards, Regina F; Ramsamooj, Rajen; deVere White, Ralph W.

In: Journal of Biomedical Optics, Vol. 9, No. 3, 05.2004, p. 587-592.

Research output: Contribution to journalArticle

@article{25aee56863e24a67962dc298288d3392,
title = "Near-infrared autofluorescence imaging for detection of cancer",
abstract = "Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation. A set of images for each tissue sample was recorded that consisted of two autofluorescence images obtained under 532- and 632.8-nm excitation and lightscattering images obtained under linearly polarized illumination at 700, 850, and 1000 nm. These images were compared with the histopathology of the tissue sample. The experimental results indicated that for various tissue types, the intensity of the autofluorescence integrated over the 700 to 1000-nm spectral region was considerably different in cancer tissues than in that of the contiguous nonneoplastic tissues. This difference provided the basis for the detection of cancer and delineation of the tumor margins. Variations on the relative intensity were observed among different tissue types and excitation wavelengths.",
keywords = "Autofluorescence, Cancer, Near-infrared",
author = "Demos, {Stavros G.} and Gandour-Edwards, {Regina F} and Rajen Ramsamooj and {deVere White}, {Ralph W}",
year = "2004",
month = "5",
doi = "10.1117/1.1688812",
language = "English (US)",
volume = "9",
pages = "587--592",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "3",

}

TY - JOUR

T1 - Near-infrared autofluorescence imaging for detection of cancer

AU - Demos, Stavros G.

AU - Gandour-Edwards, Regina F

AU - Ramsamooj, Rajen

AU - deVere White, Ralph W

PY - 2004/5

Y1 - 2004/5

N2 - Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation. A set of images for each tissue sample was recorded that consisted of two autofluorescence images obtained under 532- and 632.8-nm excitation and lightscattering images obtained under linearly polarized illumination at 700, 850, and 1000 nm. These images were compared with the histopathology of the tissue sample. The experimental results indicated that for various tissue types, the intensity of the autofluorescence integrated over the 700 to 1000-nm spectral region was considerably different in cancer tissues than in that of the contiguous nonneoplastic tissues. This difference provided the basis for the detection of cancer and delineation of the tumor margins. Variations on the relative intensity were observed among different tissue types and excitation wavelengths.

AB - Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation. A set of images for each tissue sample was recorded that consisted of two autofluorescence images obtained under 532- and 632.8-nm excitation and lightscattering images obtained under linearly polarized illumination at 700, 850, and 1000 nm. These images were compared with the histopathology of the tissue sample. The experimental results indicated that for various tissue types, the intensity of the autofluorescence integrated over the 700 to 1000-nm spectral region was considerably different in cancer tissues than in that of the contiguous nonneoplastic tissues. This difference provided the basis for the detection of cancer and delineation of the tumor margins. Variations on the relative intensity were observed among different tissue types and excitation wavelengths.

KW - Autofluorescence

KW - Cancer

KW - Near-infrared

UR - http://www.scopus.com/inward/record.url?scp=2942756060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942756060&partnerID=8YFLogxK

U2 - 10.1117/1.1688812

DO - 10.1117/1.1688812

M3 - Article

C2 - 15189097

AN - SCOPUS:2942756060

VL - 9

SP - 587

EP - 592

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 3

ER -