Abstract
Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation. A set of images for each tissue sample was recorded that consisted of two autofluorescence images obtained under 532- and 632.8-nm excitation and lightscattering images obtained under linearly polarized illumination at 700, 850, and 1000 nm. These images were compared with the histopathology of the tissue sample. The experimental results indicated that for various tissue types, the intensity of the autofluorescence integrated over the 700 to 1000-nm spectral region was considerably different in cancer tissues than in that of the contiguous nonneoplastic tissues. This difference provided the basis for the detection of cancer and delineation of the tumor margins. Variations on the relative intensity were observed among different tissue types and excitation wavelengths.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 587-592 |
| Number of pages | 6 |
| Journal | Journal of Biomedical Optics |
| Volume | 9 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 2004 |
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Keywords
- Autofluorescence
- Cancer
- Near-infrared
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
- Clinical Biochemistry
Cite this
Near-infrared autofluorescence imaging for detection of cancer. / Demos, Stavros G.; Gandour-Edwards, Regina F; Ramsamooj, Rajen; deVere White, Ralph W.
In: Journal of Biomedical Optics, Vol. 9, No. 3, 05.2004, p. 587-592.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Near-infrared autofluorescence imaging for detection of cancer
AU - Demos, Stavros G.
AU - Gandour-Edwards, Regina F
AU - Ramsamooj, Rajen
AU - deVere White, Ralph W
PY - 2004/5
Y1 - 2004/5
N2 - Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation. A set of images for each tissue sample was recorded that consisted of two autofluorescence images obtained under 532- and 632.8-nm excitation and lightscattering images obtained under linearly polarized illumination at 700, 850, and 1000 nm. These images were compared with the histopathology of the tissue sample. The experimental results indicated that for various tissue types, the intensity of the autofluorescence integrated over the 700 to 1000-nm spectral region was considerably different in cancer tissues than in that of the contiguous nonneoplastic tissues. This difference provided the basis for the detection of cancer and delineation of the tumor margins. Variations on the relative intensity were observed among different tissue types and excitation wavelengths.
AB - Near-infrared autofluorescence imaging of tissues under long-wavelength laser excitation in the green and red spectral region complemented by cross-polarized elastic light scattering was explored for cancer detection. Various types of normal and malignant human tissue samples were utilized in this investigation. A set of images for each tissue sample was recorded that consisted of two autofluorescence images obtained under 532- and 632.8-nm excitation and lightscattering images obtained under linearly polarized illumination at 700, 850, and 1000 nm. These images were compared with the histopathology of the tissue sample. The experimental results indicated that for various tissue types, the intensity of the autofluorescence integrated over the 700 to 1000-nm spectral region was considerably different in cancer tissues than in that of the contiguous nonneoplastic tissues. This difference provided the basis for the detection of cancer and delineation of the tumor margins. Variations on the relative intensity were observed among different tissue types and excitation wavelengths.
KW - Autofluorescence
KW - Cancer
KW - Near-infrared
UR - http://www.scopus.com/inward/record.url?scp=2942756060&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=2942756060&partnerID=8YFLogxK
U2 - 10.1117/1.1688812
DO - 10.1117/1.1688812
M3 - Article
C2 - 15189097
AN - SCOPUS:2942756060
VL - 9
SP - 587
EP - 592
JO - Journal of Biomedical Optics
JF - Journal of Biomedical Optics
SN - 1083-3668
IS - 3
ER -