Ndj1, a telomere-associated protein, promotes meiotic recombination in budding yeast

Hsin Yen Wu, Sean M. Burgess

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Dynamic telomere repositioning is a prominent feature of meiosis. Deletion of a telomere-associated protein, Ndj1, results in the failure of both attachment and clustering of telomeres at the nuclear envelope and delays several landmarks of meiosis I, such as pairing, synaptonemal complex formation, and timing of the meiosis I division. We explored the role of Ndj1 in meiotic recombination, which occurs through the formation and repair of programmed double-strand breaks. The ndj1Δ mutation allows for the formation of the first detectable strand invasion intermediate (i.e., single-end invasion) with wild-type kinetics; however, it confers a delay in the formation of the double-Holliday junction intermediate and both crossover and noncrossover products. These results challenge the widely held notion that clustering of telomeres in meiosis promotes the ability of homologous chromosomes to find one another in budding Saccharomyces cerevisiae. We propose that an Ndj1-dependent function is critical for stabilizing analogous strand invasion intermediates that exist in two separate branches of the bifurcated pathway, leading to either noncrossover or crossover formation. These findings provide a link between telomere dynamics and a distinct mechanistic step of meiotic recombination that follows the homology search.

Original languageEnglish (US)
Pages (from-to)3683-3694
Number of pages12
JournalMolecular and Cellular Biology
Volume26
Issue number10
DOIs
StatePublished - May 2006

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Saccharomycetales
Telomere
Genetic Recombination
Meiosis
Proteins
Cluster Analysis
Cruciform DNA
Synaptonemal Complex
Nuclear Envelope
Saccharomyces cerevisiae
Chromosomes
Mutation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Ndj1, a telomere-associated protein, promotes meiotic recombination in budding yeast. / Wu, Hsin Yen; Burgess, Sean M.

In: Molecular and Cellular Biology, Vol. 26, No. 10, 05.2006, p. 3683-3694.

Research output: Contribution to journalArticle

Wu, Hsin Yen ; Burgess, Sean M. / Ndj1, a telomere-associated protein, promotes meiotic recombination in budding yeast. In: Molecular and Cellular Biology. 2006 ; Vol. 26, No. 10. pp. 3683-3694.
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