Natural bovine osteogenin and recombinant human bone morphogenetic protein-2B are equipotent in the maintenance of proteoglycans in bovine articular cartilage expiant cultures

Frank P. Luyten, Yu M. Yu, Masaki Yanagishita, Slobodan Vukicevic, R. Glenn Hammonds, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

135 Scopus citations

Abstract

Osteogenin and related bone morphogenetic proteins are members of the transforming growth factor-β superfamily, and were isolated by their ability to induce cartilage and bone formation in vivo. The influence of osteogenin, purified from bovine bone, and of recombinant human bone morphogenetic protein-2B (BMP-28) has been examined in bovine articular cartilage expiants. Both differentiation factors stimulated in a dose-dependent manner the synthesis of proteoglycans and decreased their rate of degradation. At a dose of 30 ng/ml, proteoglycan synthesis was increased to levels observed with either 20 ng/ml insulin-like growth factor I, 10 ng/ml transforming growth factor-β, or 20% fetal bovine serum. This increase of biosynthetic rates above basal medium levels was observed in young, adolescent, and adult tissues. Analysis of the size of the newly synthesized proteoglycans, the glycosaminoglycan chain size, and the glycosaminoglycan type of explants treated with osteogenin or BMP-2B were very comparable to each other, and to proteoglycans isolated from cartilage treated with either insulin-like growth factor I or fetal bovine serum. These results demonstrate that osteogenin and BMP-2B alone are capable of stimulating and maintaining the chondrocyte phenotype in vitro.

Original languageEnglish (US)
Pages (from-to)3691-3695
Number of pages5
JournalJournal of Biological Chemistry
Volume267
Issue number6
StatePublished - Feb 25 1992
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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