Natural antibody contributes to host defense against an attenuated Brucella abortus virB mutant

Hortensia G. Rolán, Mariana N. Xavier, Renato L. Santos, Renee M Tsolis

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Brucella abortus is an intracellular pathogen that persists within phagocytic cells of the reticuloendothelial system. To identify in vivo interactions between B. abortus and the host that lead to persistent infection, we studied the persistence of B. abortus and an isogenic virB mutant deficient in the VirB type IV secretion system (T4SS) in knockout mice. In contrast to control mice, mice lacking B cells (Igh6-/-) were permissive for infection with the attenuated virB mutant. To determine the basis for this phenotype, we characterized immune functions of Igh6-/- mice in the context of B. abortus infection. Igh6-/- mice had greater numbers of extracellular bacteria in the spleen and increased early expression of proinflammatory cytokines during B. abortus infection. Further, a virB mutant, despite its wild-type level of survival, failed to elicit microgranuloma formation in the spleens of Igh6-/- mice, suggesting a requirement for the T4SS to elicit this pathological change. Passive transfer of immunoglobulin G from naïve mice restored the ability of Igh6-/- mice to control the persistence of the virB mutant by a complement-independent mechanism. Further, adoptive transfer of CD11b+ cells from C57BL/6 mice to Igh6-/- mice restored the ability of the knockout mice to limit the replication of the virB mutant in the spleen, suggesting that the Igh6-/- mutation affects phagocyte function and that phagocyte function can be restored by natural antibody.

Original languageEnglish (US)
Pages (from-to)3004-3013
Number of pages10
JournalInfection and Immunity
Volume77
Issue number7
DOIs
StatePublished - Jul 2009

Fingerprint

Brucella abortus
Antibodies
Phagocytes
Spleen
Infection
Knockout Mice
Mononuclear Phagocyte System
Adoptive Transfer
Inbred C57BL Mouse
B-Lymphocytes
Immunoglobulin G
Cytokines
Bacteria
Phenotype
Mutation

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Natural antibody contributes to host defense against an attenuated Brucella abortus virB mutant. / Rolán, Hortensia G.; Xavier, Mariana N.; Santos, Renato L.; Tsolis, Renee M.

In: Infection and Immunity, Vol. 77, No. 7, 07.2009, p. 3004-3013.

Research output: Contribution to journalArticle

Rolán, Hortensia G. ; Xavier, Mariana N. ; Santos, Renato L. ; Tsolis, Renee M. / Natural antibody contributes to host defense against an attenuated Brucella abortus virB mutant. In: Infection and Immunity. 2009 ; Vol. 77, No. 7. pp. 3004-3013.
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