Native/Denaturing two-dimensional DNA electrophoresis and its application to the analysis of recombination intermediates

Jessica P. Lao; Shangming Tang; Neil Hunter

doi:10.1007/978-1-62703-565-1_6

Native/Denaturing two-dimensional DNA electrophoresis and its application to the analysis of recombination intermediates

Jessica P. Lao, Shangming Tang, Neil Hunter

Cell Biology and Human Anatomy

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Two-dimensional (2D) gel electrophoresis employs distinct electrophoretic conditions to better resolve complex mixtures of molecules. In combination with Southern analysis, 2D agarose gel electrophoresis is routinely employed to detect and analyze DNA intermediates that arise during the replication and repair of chromosomes. By separating intermediates into their component single-strands, native/denaturing 2D gels can reveal structure that is not apparent under native conditions alone. Here, we describe a general method for native/denaturing two-dimensional gel electrophoresis and its application to understanding the DNA strand-composition of recombination intermediates formed during meiosis.

Original language	English (US)
Pages (from-to)	105-120
Number of pages	16
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	1054
DOIs	https://doi.org/10.1007/978-1-62703-565-1_6
State	Published - 2013

ASJC Scopus subject areas

Medicine(all)

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