Native or proteolytically activated NanI sialidase enhances the binding and cytotoxic activity of Clostridium perfringens enterotoxin and beta toxin

James R. Theoret, Jihong Li, Mauricio A. Navarro, Jorge P. Garcia, Francisco A Uzal, Bruce A. McClane

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Many Clostridium perfringens strains produce NanI as their major sialidase. Previous studies showed that NanI could potentiate C. perfringens epsilon toxin cytotoxicity by enhancing the binding of this toxin to host cells. The present study first determined that NanI exerts similar cytotoxicity-enhancing effects on C. perfringens enterotoxin and beta toxin, which are also important toxins for C. perfringens diseases (enteritis and enterotoxemia) originating in the gastrointestinal (GI) tract. Building upon previous work demonstrating that purified trypsin can activate NanI activity, this study next determined that purified chymotrypsin or mouse intestinal fluids can also activate NanI activity. Amino acid sequencing then showed that this effect involves the N-terminal processing of the NanI protein. Recombinant NanI (rNanI) species corresponding to major chymotrypsin- or small intestinal fluid-generated NanI fragments possessed more sialidase activity than did full-length rNanI, further supporting the proteolytic activation of NanI activity. rNanI species corresponding to proteolysis products also promoted the cytotoxic activity and binding of enterotoxin and beta toxin more strongly than did full-length rNanI. Since enterotoxin and beta toxin are produced in the intestines during human and animal disease, these findings suggest that intestinal proteases may enhance NanI activity, which in turn could further potentiate the activity of intestinally active toxins during disease. Coupling these new results with previous findings demonstrating that NanI is important for the adherence of C. perfringens to enterocyte-like cells, NanI sialidase is now emerging as a potential auxiliary virulence factor for C. perfringens enteritis and enterotoxemia.

Original languageEnglish (US)
Article numbere00730-17
JournalInfection and Immunity
Volume86
Issue number1
DOIs
StatePublished - Jan 1 2018

Fingerprint

Clostridium perfringens
Neuraminidase
Enterotoxemia
Enteritis
Enterotoxins
Chymotrypsin
Animal Diseases
Enterocytes
Protein Sequence Analysis
Virulence Factors
Trypsin
Proteolysis
Intestines
Gastrointestinal Tract
Peptide Hydrolases
Clostridium perfringens CPB protein
Clostridium enterotoxin
Proteins

Keywords

  • Beta toxin
  • Clostridium perfringens
  • Enterotoxin
  • Sialidase

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Native or proteolytically activated NanI sialidase enhances the binding and cytotoxic activity of Clostridium perfringens enterotoxin and beta toxin. / Theoret, James R.; Li, Jihong; Navarro, Mauricio A.; Garcia, Jorge P.; Uzal, Francisco A; McClane, Bruce A.

In: Infection and Immunity, Vol. 86, No. 1, e00730-17, 01.01.2018.

Research output: Contribution to journalArticle

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