The detection of autoantibody/antigen recognition depends on the specific interaction between the antibody and its epitope. In most cases, recombinant autoantigens produced in E. coli are convenient and reliable sources of antigen that can be readily applied in detection systems such as Enzyme-linked immunosorbent assay (ELISA), immunoblot and microarray which offers sensitive, specific and high throughput assays. The isolation of native autoantigens from natural sources such as animal tissues faces major limitations with respect to yield, reproducibility and purity. In light of increasing number of autoantigens identified at the molecular level and knowledge on their epitopes as well as improved molecular biological methods in producing recombinant autoantigens from cloned genes, recombinant autoantigens have become powerful reagents in the analysis of the antibody/antigen interaction. However, in cases when the proper post-translational modification and protein folding is necessary for antibody recognition, one has to seek the use of native autoantigens that are purified from mammalian tissues or recombinant proteins produced in eukaryotic cells are necessary. Thus, the appropriate choice of laboratory assays depends on the knowledge of the specific antibody/antigen interaction and it is important to be familiar with the limitations of the technology currently in use in your local laboratory.
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)