Nasal immunization of nonhuman primates with simian immunodeficiency virus p55(gag) and cholera toxin adjuvant induces Th1/Th2 help for virus- specific immune responses in reproductive tissues

Koichi Imaoka, Chris J Miller, Mitsuru Kubota, Michael B. McChesney, Barbara Lohman, Masafumi Yamamoto, Kohtaro Fujihashi, Kenji Someya, Mitsuo Honda, Jerry R. McGhee, Hiroshi Kiyono

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

Female rhesus macaques were nasally immunized with p55(gag) (p55) of SIV and cholera toxin as a mucosal adjuvant. Nasal immunization induced Ag- specific IgA and IgG Abs in mucosal secretions (e.g., cervicovaginal secretions, rectal washes, and saliva) and serum. Furthermore, high numbers of p55-specific IgA and IgG Ab-forming cells were induced in mucosal effector sites, i.e., uterine cervix, intestinal lamina propria, and nasal passage. p55-specific CD4+ T cells in both systemic and mucosal compartments expressed IFN-γ and IL-2 (Th1-type)- as well as IL-5, IL-6, and IL-10 (Th2- type)-specific mRNA. Moreover, p55-specific CTL activity was demonstrated in lymphocytes from blood, tonsils, and other lymphoid tissues. These results show that nasal immunization with SIV p55 with cholera toxin elicits both Th1- and selective Th2-type cytokine responses associated with the induction of SIV-specific mucosal and serum Abs, and CTL activity. These results offer a promise for the development of protective mucosal immunity to SIV.

Original languageEnglish (US)
Pages (from-to)5952-5958
Number of pages7
JournalJournal of Immunology
Volume161
Issue number11
StatePublished - Dec 1 1998

ASJC Scopus subject areas

  • Immunology

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    Imaoka, K., Miller, C. J., Kubota, M., McChesney, M. B., Lohman, B., Yamamoto, M., Fujihashi, K., Someya, K., Honda, M., McGhee, J. R., & Kiyono, H. (1998). Nasal immunization of nonhuman primates with simian immunodeficiency virus p55(gag) and cholera toxin adjuvant induces Th1/Th2 help for virus- specific immune responses in reproductive tissues. Journal of Immunology, 161(11), 5952-5958.