TY - JOUR
T1 - Nasal immunization of nonhuman primates with simian immunodeficiency virus p55(gag) and cholera toxin adjuvant induces Th1/Th2 help for virus- specific immune responses in reproductive tissues
AU - Imaoka, Koichi
AU - Miller, Chris J
AU - Kubota, Mitsuru
AU - McChesney, Michael B.
AU - Lohman, Barbara
AU - Yamamoto, Masafumi
AU - Fujihashi, Kohtaro
AU - Someya, Kenji
AU - Honda, Mitsuo
AU - McGhee, Jerry R.
AU - Kiyono, Hiroshi
PY - 1998/12/1
Y1 - 1998/12/1
N2 - Female rhesus macaques were nasally immunized with p55(gag) (p55) of SIV and cholera toxin as a mucosal adjuvant. Nasal immunization induced Ag- specific IgA and IgG Abs in mucosal secretions (e.g., cervicovaginal secretions, rectal washes, and saliva) and serum. Furthermore, high numbers of p55-specific IgA and IgG Ab-forming cells were induced in mucosal effector sites, i.e., uterine cervix, intestinal lamina propria, and nasal passage. p55-specific CD4+ T cells in both systemic and mucosal compartments expressed IFN-γ and IL-2 (Th1-type)- as well as IL-5, IL-6, and IL-10 (Th2- type)-specific mRNA. Moreover, p55-specific CTL activity was demonstrated in lymphocytes from blood, tonsils, and other lymphoid tissues. These results show that nasal immunization with SIV p55 with cholera toxin elicits both Th1- and selective Th2-type cytokine responses associated with the induction of SIV-specific mucosal and serum Abs, and CTL activity. These results offer a promise for the development of protective mucosal immunity to SIV.
AB - Female rhesus macaques were nasally immunized with p55(gag) (p55) of SIV and cholera toxin as a mucosal adjuvant. Nasal immunization induced Ag- specific IgA and IgG Abs in mucosal secretions (e.g., cervicovaginal secretions, rectal washes, and saliva) and serum. Furthermore, high numbers of p55-specific IgA and IgG Ab-forming cells were induced in mucosal effector sites, i.e., uterine cervix, intestinal lamina propria, and nasal passage. p55-specific CD4+ T cells in both systemic and mucosal compartments expressed IFN-γ and IL-2 (Th1-type)- as well as IL-5, IL-6, and IL-10 (Th2- type)-specific mRNA. Moreover, p55-specific CTL activity was demonstrated in lymphocytes from blood, tonsils, and other lymphoid tissues. These results show that nasal immunization with SIV p55 with cholera toxin elicits both Th1- and selective Th2-type cytokine responses associated with the induction of SIV-specific mucosal and serum Abs, and CTL activity. These results offer a promise for the development of protective mucosal immunity to SIV.
UR - http://www.scopus.com/inward/record.url?scp=0032402228&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032402228&partnerID=8YFLogxK
M3 - Article
C2 - 9834076
AN - SCOPUS:0032402228
VL - 161
SP - 5952
EP - 5958
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 11
ER -