Nasal immunization of nonhuman primates with simian immunodeficiency virus p55(gag) and cholera toxin adjuvant induces Th1/Th2 help for virus- specific immune responses in reproductive tissues

Koichi Imaoka, Chris J Miller, Mitsuru Kubota, Michael B. McChesney, Barbara Lohman, Masafumi Yamamoto, Kohtaro Fujihashi, Kenji Someya, Mitsuo Honda, Jerry R. McGhee, Hiroshi Kiyono

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Abstract

Female rhesus macaques were nasally immunized with p55(gag) (p55) of SIV and cholera toxin as a mucosal adjuvant. Nasal immunization induced Ag- specific IgA and IgG Abs in mucosal secretions (e.g., cervicovaginal secretions, rectal washes, and saliva) and serum. Furthermore, high numbers of p55-specific IgA and IgG Ab-forming cells were induced in mucosal effector sites, i.e., uterine cervix, intestinal lamina propria, and nasal passage. p55-specific CD4+ T cells in both systemic and mucosal compartments expressed IFN-γ and IL-2 (Th1-type)- as well as IL-5, IL-6, and IL-10 (Th2- type)-specific mRNA. Moreover, p55-specific CTL activity was demonstrated in lymphocytes from blood, tonsils, and other lymphoid tissues. These results show that nasal immunization with SIV p55 with cholera toxin elicits both Th1- and selective Th2-type cytokine responses associated with the induction of SIV-specific mucosal and serum Abs, and CTL activity. These results offer a promise for the development of protective mucosal immunity to SIV.

Original languageEnglish (US)
Pages (from-to)5952-5958
Number of pages7
JournalJournal of Immunology
Volume161
Issue number11
StatePublished - Dec 1 1998

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Cholera Toxin
Nose
Primates
Immunization
Viruses
Immunoglobulin A
Immunoglobulin G
Mucosal Immunity
Palatine Tonsil
Interleukin-5
Lymphoid Tissue
Macaca mulatta
Serum
Saliva
Cervix Uteri
Interleukin-10
Interleukin-2
Interleukin-6
Mucous Membrane
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Nasal immunization of nonhuman primates with simian immunodeficiency virus p55(gag) and cholera toxin adjuvant induces Th1/Th2 help for virus- specific immune responses in reproductive tissues. / Imaoka, Koichi; Miller, Chris J; Kubota, Mitsuru; McChesney, Michael B.; Lohman, Barbara; Yamamoto, Masafumi; Fujihashi, Kohtaro; Someya, Kenji; Honda, Mitsuo; McGhee, Jerry R.; Kiyono, Hiroshi.

In: Journal of Immunology, Vol. 161, No. 11, 01.12.1998, p. 5952-5958.

Research output: Contribution to journalArticle

Imaoka, K, Miller, CJ, Kubota, M, McChesney, MB, Lohman, B, Yamamoto, M, Fujihashi, K, Someya, K, Honda, M, McGhee, JR & Kiyono, H 1998, 'Nasal immunization of nonhuman primates with simian immunodeficiency virus p55(gag) and cholera toxin adjuvant induces Th1/Th2 help for virus- specific immune responses in reproductive tissues', Journal of Immunology, vol. 161, no. 11, pp. 5952-5958.
Imaoka, Koichi ; Miller, Chris J ; Kubota, Mitsuru ; McChesney, Michael B. ; Lohman, Barbara ; Yamamoto, Masafumi ; Fujihashi, Kohtaro ; Someya, Kenji ; Honda, Mitsuo ; McGhee, Jerry R. ; Kiyono, Hiroshi. / Nasal immunization of nonhuman primates with simian immunodeficiency virus p55(gag) and cholera toxin adjuvant induces Th1/Th2 help for virus- specific immune responses in reproductive tissues. In: Journal of Immunology. 1998 ; Vol. 161, No. 11. pp. 5952-5958.
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abstract = "Female rhesus macaques were nasally immunized with p55(gag) (p55) of SIV and cholera toxin as a mucosal adjuvant. Nasal immunization induced Ag- specific IgA and IgG Abs in mucosal secretions (e.g., cervicovaginal secretions, rectal washes, and saliva) and serum. Furthermore, high numbers of p55-specific IgA and IgG Ab-forming cells were induced in mucosal effector sites, i.e., uterine cervix, intestinal lamina propria, and nasal passage. p55-specific CD4+ T cells in both systemic and mucosal compartments expressed IFN-γ and IL-2 (Th1-type)- as well as IL-5, IL-6, and IL-10 (Th2- type)-specific mRNA. Moreover, p55-specific CTL activity was demonstrated in lymphocytes from blood, tonsils, and other lymphoid tissues. These results show that nasal immunization with SIV p55 with cholera toxin elicits both Th1- and selective Th2-type cytokine responses associated with the induction of SIV-specific mucosal and serum Abs, and CTL activity. These results offer a promise for the development of protective mucosal immunity to SIV.",
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