Naphthalene metabolism by human lung microsomal enzymes

Alan R Buckpitt, Linda S. Bahnson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In the presence of glutathione and glutathione transferases, microsomal fractions prepared from fresh samples of human lung tissue obtained at resection metabolized naphthalene to naphthalene dihydrodiol and 3 glutathione conjugates at easily measurable rates. Addition of varying amounts of human lung microsomal protein markedly inhibited mouse liver microsome-catalyzed naphthalene metabolism in one sample but not the other. These data show that naphthalene is a good substrate for human pulmonary microsomal monooxygenases. In addition, these studies suggest that there may be an inhibitor, potentially released during tissue homogenization, that makes measurement of human lung xenobiotic metabolism difficult.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalToxicology
Volume41
Issue number3
DOIs
StatePublished - 1986

Fingerprint

Metabolism
Lung
Enzymes
Glutathione
Tissue
Aryl Hydrocarbon Hydroxylases
Liver Microsomes
Xenobiotics
Glutathione Transferase
Liver
naphthalene
Substrates
Proteins

Keywords

  • Cytochrome P-450
  • Glutathione conjugates
  • Human
  • Metabolism
  • Naphthalene

ASJC Scopus subject areas

  • Toxicology

Cite this

Naphthalene metabolism by human lung microsomal enzymes. / Buckpitt, Alan R; Bahnson, Linda S.

In: Toxicology, Vol. 41, No. 3, 1986, p. 333-341.

Research output: Contribution to journalArticle

Buckpitt, Alan R ; Bahnson, Linda S. / Naphthalene metabolism by human lung microsomal enzymes. In: Toxicology. 1986 ; Vol. 41, No. 3. pp. 333-341.
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