Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation

Dokyoon Kim, Francesco Marchetti, Zuxiong Chen, Sasa Zaric, Robert J. Wilson, Drew A. Hall, Richard S. Gaster, Jung Rok Lee, Junyi Wang, Sebastian J. Osterfeld, Heng Yu, Robert M. White, William F. Blakely, Leif E. Peterson, Sandhya Bhatnagar, Brandon Mannion, Serena Tseng, Kristen Roth, Matthew A Coleman, Antoine M. SnijdersAndrew J. Wyrobek, Shan X. Wang

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Giant magnetoresistive (GMR) nanosensors provide a novel approach for measuring protein concentrations in blood for medical diagnosis. Using an in vivo mouse radiation model, we developed protocols for measuring Flt3 ligand (Flt3lg) and serum amyloid A1 (Saa1) in small amounts of blood collected during the first week after X-ray exposures of sham, 0.1, 1, 2, 3, or 6 Gy. Flt3lg concentrations showed excellent dose discrimination at ≥ 1 Gy in the time window of 1 to 7 days after exposure except 1 Gy at day 7. Saa1 dose response was limited to the first two days after exposure. A multiplex assay with both proteins showed improved dose classification accuracy. Our magneto-nanosensor assay demonstrates the dose and time responses, low-dose sensitivity, small volume requirements, and rapid speed that have important advantages in radiation triage biodosimetry.

Original languageEnglish (US)
Article number2234
JournalScientific Reports
Volume3
DOIs
StatePublished - 2013
Externally publishedYes

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ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Kim, D., Marchetti, F., Chen, Z., Zaric, S., Wilson, R. J., Hall, D. A., Gaster, R. S., Lee, J. R., Wang, J., Osterfeld, S. J., Yu, H., White, R. M., Blakely, W. F., Peterson, L. E., Bhatnagar, S., Mannion, B., Tseng, S., Roth, K., Coleman, M. A., ... Wang, S. X. (2013). Nanosensor dosimetry of mouse blood proteins after exposure to ionizing radiation. Scientific Reports, 3, [2234]. https://doi.org/10.1038/srep02234