Nanosecond pulsed electric fields trigger intracellular signals in human lymphocytes

P. T. Vernier, Y. Sun, Laura Marcu, C. M. Craft, M. A. Gundersen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

We present evidence for a new technological approach to signaling and triggering of intracellular events in biological cells. Remotely applied nanosecond, megavoltper-meter, pulsed electric fields perturb the intracellular environment, causing calcium release, phosphatidylserine externalization, and apoptosis. This technology does not require direct electrical contacts to biological material. Ultra-short pulses with durations less than the charging time constant of the plasma membrane generate voltages across structures in the cell interior, enabling intracellular manipulations with stimuli that are remotely generated and controlled. The work reported here suggests possibilities within the reach of state-of-the-art bioelectrical engineering and nanotechnology for utilizing nanopulse-triggered intracellular electroperturbations in remote bio-systems deployed as reporters and effectors, and for other applications such as wound healing.

Original languageEnglish (US)
Title of host publication2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
EditorsM. Laudon, B. Romanowicz
Pages7-10
Number of pages4
Volume1
StatePublished - 2004
Event2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 - Boston, MA, United States
Duration: Mar 7 2004Mar 11 2004

Other

Other2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004
CountryUnited States
CityBoston, MA
Period3/7/043/11/04

Keywords

  • Calcium burst
  • Electroperturbation
  • Megavolt-per-meter
  • Nanosecond
  • Phosphatidylserine translocation

ASJC Scopus subject areas

  • Engineering(all)

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  • Cite this

    Vernier, P. T., Sun, Y., Marcu, L., Craft, C. M., & Gundersen, M. A. (2004). Nanosecond pulsed electric fields trigger intracellular signals in human lymphocytes. In M. Laudon, & B. Romanowicz (Eds.), 2004 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2004 (Vol. 1, pp. 7-10)