Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts

P. Thomas Vernier, Yinghua Sun, Laura Marcu, Cheryl M. Craft, Martin A. Gundersen

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalFEBS Letters
Volume572
Issue number1-3
DOIs
StatePublished - Aug 13 2004

Fingerprint

Phosphatidylserines
Phospholipids
Electrodes
Electric fields
Membranes
Cell membranes
Poles
Anodes
Cell Membrane
Direction compound

Keywords

  • BAPTA, 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid
  • Nanosecond pulsed electric field
  • Phosphatidylserine externalization
  • Phospholipid translocation
  • Real-time fluorescence microscopy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Vernier, P. T., Sun, Y., Marcu, L., Craft, C. M., & Gundersen, M. A. (2004). Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts. FEBS Letters, 572(1-3), 103-108. https://doi.org/10.1016/j.febslet.2004.07.021

Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts. / Vernier, P. Thomas; Sun, Yinghua; Marcu, Laura; Craft, Cheryl M.; Gundersen, Martin A.

In: FEBS Letters, Vol. 572, No. 1-3, 13.08.2004, p. 103-108.

Research output: Contribution to journalArticle

Vernier, PT, Sun, Y, Marcu, L, Craft, CM & Gundersen, MA 2004, 'Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts', FEBS Letters, vol. 572, no. 1-3, pp. 103-108. https://doi.org/10.1016/j.febslet.2004.07.021
Vernier, P. Thomas ; Sun, Yinghua ; Marcu, Laura ; Craft, Cheryl M. ; Gundersen, Martin A. / Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts. In: FEBS Letters. 2004 ; Vol. 572, No. 1-3. pp. 103-108.
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