Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts

P. Thomas Vernier; Yinghua Sun; Laura Marcu; Cheryl M. Craft; Martin A. Gundersen

doi:10.1016/j.febslet.2004.07.021

Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts

P. Thomas Vernier, Yinghua Sun, Laura Marcu, Cheryl M. Craft, Martin A. Gundersen

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.

Original language	English (US)
Pages (from-to)	103-108
Number of pages	6
Journal	FEBS Letters
Volume	572
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.07.021
State	Published - Aug 13 2004

Keywords

BAPTA, 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid
Nanosecond pulsed electric field
Phosphatidylserine externalization
Phospholipid translocation
Real-time fluorescence microscopy

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

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10.1016/j.febslet.2004.07.021

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title = "Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts",

abstract = "Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.",

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T1 - Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts

AU - Vernier, P. Thomas

AU - Sun, Yinghua

AU - Marcu, Laura

AU - Craft, Cheryl M.

AU - Gundersen, Martin A.

PY - 2004/8/13

Y1 - 2004/8/13

N2 - Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.

AB - Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.

KW - BAPTA, 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid

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KW - Phospholipid translocation

KW - Real-time fluorescence microscopy

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Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts

Abstract

Keywords

ASJC Scopus subject areas

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