Abstract
Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 103-108 |
| Number of pages | 6 |
| Journal | FEBS Letters |
| Volume | 572 |
| Issue number | 1-3 |
| DOIs | |
| State | Published - Aug 13 2004 |
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Keywords
- BAPTA, 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid
- Nanosecond pulsed electric field
- Phosphatidylserine externalization
- Phospholipid translocation
- Real-time fluorescence microscopy
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts. / Vernier, P. Thomas; Sun, Yinghua; Marcu, Laura; Craft, Cheryl M.; Gundersen, Martin A.
In: FEBS Letters, Vol. 572, No. 1-3, 13.08.2004, p. 103-108.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nanosecond pulsed electric fields perturb membrane phospholipids in T lymphoblasts
AU - Vernier, P. Thomas
AU - Sun, Yinghua
AU - Marcu, Laura
AU - Craft, Cheryl M.
AU - Gundersen, Martin A.
PY - 2004/8/13
Y1 - 2004/8/13
N2 - Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.
AB - Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in cell membranes without the permeabilization associated with longer, lower-field pulses. A single 30 ns, 2.5 MV/m pulse produces perturbations consistent with phosphatidylserine (PS) externalization in Jurkat T lymphoblasts within milliseconds, polarized in the direction of the applied field, indicating an immediate interaction between membrane components and the electric field. This disturbance occurs only at the anode pole of the cell, supporting the hypothesis that the pulsed field drives the negatively charged PS head group toward the positive electrode, directly providing the energy for crossing the membrane dielectric barrier.
KW - BAPTA, 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid
KW - Nanosecond pulsed electric field
KW - Phosphatidylserine externalization
KW - Phospholipid translocation
KW - Real-time fluorescence microscopy
UR - http://www.scopus.com/inward/record.url?scp=4143050399&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=4143050399&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2004.07.021
DO - 10.1016/j.febslet.2004.07.021
M3 - Article
C2 - 15304332
AN - SCOPUS:4143050399
VL - 572
SP - 103
EP - 108
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1-3
ER -