Nanoscale fluoro-immuno assays with lanthanide oxide nanoparticles

I. M. Kennedy, M. Koivunen, S. Gee, C. Cummins, R. Perron, D. Dosev, B. D. Hammock

Research output: Chapter in Book/Report/Conference proceedingConference contribution

4 Scopus citations

Abstract

The use of polystyrene nanoparticles with europium chelate has been demonstrated as fluorescent reporters in an immunoassay for atrazine. The limit of detection with the nanoparticles was similar to that achieved with a conventional ELISA. It was shown that as the particle size decreased the time required for binding decreased and the sensitivity of the assay increased. This suggests that the use of smaller particles would greatly speed up the reaction and simultaneously increase sensitivity. However, the detection system used sets limits to the particle size as well. There is clearly a point where our detection system would not be sensitive enough to detect the emission from small particles. Therefore, a highly sensitive excitation/detection system needs to be developed to fully utilize the kinetic advantage from small particle size.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsM.S. Islam, A.K. Dutta
Pages329-339
Number of pages11
Volume5593
DOIs
StatePublished - 2004
EventNanosensing Materials and Devices - Philadelphia, PA, United States
Duration: Oct 25 2004Oct 28 2004

Other

OtherNanosensing Materials and Devices
CountryUnited States
CityPhiladelphia, PA
Period10/25/0410/28/04

    Fingerprint

Keywords

  • Atrazine
  • Fluorescence
  • Immunoassay
  • Lanthanide
  • Nanoparticles

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Kennedy, I. M., Koivunen, M., Gee, S., Cummins, C., Perron, R., Dosev, D., & Hammock, B. D. (2004). Nanoscale fluoro-immuno assays with lanthanide oxide nanoparticles. In M. S. Islam, & A. K. Dutta (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 5593, pp. 329-339). [53] https://doi.org/10.1117/12.571483