Nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary

Rebecca L. Beer, Bruce W. Draper

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Female zebrafish have a prolific reproductive capacity, suggesting that a germline stem cell (GSC) population drives oocyte production. However, a zebrafish female GSC population has yet to be identified. Adult stem cells are defined by their ability to both self-renew and differentiate, and by their localization to a stem cell niche. We show here that mitotic and early meiotic germ cells are present in the adult ovary and that the zebrafish homolog of the conserved vertebrate GSC marker, nanos2, is expressed in a subset of pre-meiotic oogonia in the adult gonad. We propose that these nanos2+ cells are GSCs. Importantly, we find that mitotic, nanos2+, and early meiotic germ cells localize to the germinal zone, thus identifying this region as the probable ovarian GSC niche in zebrafish. nanos3, which encodes a conserved RNA-binding protein, is known to be required for the continued production of oocytes in the zebrafish. Although mammalian homologs of nanos3 are expressed in early spermatogonia, no study has defined the role of nanos3 in the regulation of vertebrate GSCs. Here we demonstrate that nanos3 function is required for the maintenance of GSCs, but not for their specification, and propose that nanos2 and nanos3 are partially redundant in this role.

Original languageEnglish (US)
Pages (from-to)308-318
Number of pages11
JournalDevelopmental Biology
Volume374
Issue number2
DOIs
StatePublished - Feb 15 2013

Fingerprint

Zebrafish
Ovary
Stem Cells
Stem Cell Niche
Genes
Germ Cells
Oocytes
Vertebrates
Oogonia
Spermatogonia
Adult Stem Cells
RNA-Binding Proteins
Gonads
Population
Maintenance
Oogonial Stem Cells

Keywords

  • Germline stem cells
  • Nanos2
  • Nanos3
  • Ovary
  • Stem cell niche
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology
  • Molecular Biology

Cite this

Nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary. / Beer, Rebecca L.; Draper, Bruce W.

In: Developmental Biology, Vol. 374, No. 2, 15.02.2013, p. 308-318.

Research output: Contribution to journalArticle

@article{08d8880fad9a457c9180810ba4796a84,
title = "Nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary",
abstract = "Female zebrafish have a prolific reproductive capacity, suggesting that a germline stem cell (GSC) population drives oocyte production. However, a zebrafish female GSC population has yet to be identified. Adult stem cells are defined by their ability to both self-renew and differentiate, and by their localization to a stem cell niche. We show here that mitotic and early meiotic germ cells are present in the adult ovary and that the zebrafish homolog of the conserved vertebrate GSC marker, nanos2, is expressed in a subset of pre-meiotic oogonia in the adult gonad. We propose that these nanos2+ cells are GSCs. Importantly, we find that mitotic, nanos2+, and early meiotic germ cells localize to the germinal zone, thus identifying this region as the probable ovarian GSC niche in zebrafish. nanos3, which encodes a conserved RNA-binding protein, is known to be required for the continued production of oocytes in the zebrafish. Although mammalian homologs of nanos3 are expressed in early spermatogonia, no study has defined the role of nanos3 in the regulation of vertebrate GSCs. Here we demonstrate that nanos3 function is required for the maintenance of GSCs, but not for their specification, and propose that nanos2 and nanos3 are partially redundant in this role.",
keywords = "Germline stem cells, Nanos2, Nanos3, Ovary, Stem cell niche, Zebrafish",
author = "Beer, {Rebecca L.} and Draper, {Bruce W.}",
year = "2013",
month = "2",
day = "15",
doi = "10.1016/j.ydbio.2012.12.003",
language = "English (US)",
volume = "374",
pages = "308--318",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary

AU - Beer, Rebecca L.

AU - Draper, Bruce W.

PY - 2013/2/15

Y1 - 2013/2/15

N2 - Female zebrafish have a prolific reproductive capacity, suggesting that a germline stem cell (GSC) population drives oocyte production. However, a zebrafish female GSC population has yet to be identified. Adult stem cells are defined by their ability to both self-renew and differentiate, and by their localization to a stem cell niche. We show here that mitotic and early meiotic germ cells are present in the adult ovary and that the zebrafish homolog of the conserved vertebrate GSC marker, nanos2, is expressed in a subset of pre-meiotic oogonia in the adult gonad. We propose that these nanos2+ cells are GSCs. Importantly, we find that mitotic, nanos2+, and early meiotic germ cells localize to the germinal zone, thus identifying this region as the probable ovarian GSC niche in zebrafish. nanos3, which encodes a conserved RNA-binding protein, is known to be required for the continued production of oocytes in the zebrafish. Although mammalian homologs of nanos3 are expressed in early spermatogonia, no study has defined the role of nanos3 in the regulation of vertebrate GSCs. Here we demonstrate that nanos3 function is required for the maintenance of GSCs, but not for their specification, and propose that nanos2 and nanos3 are partially redundant in this role.

AB - Female zebrafish have a prolific reproductive capacity, suggesting that a germline stem cell (GSC) population drives oocyte production. However, a zebrafish female GSC population has yet to be identified. Adult stem cells are defined by their ability to both self-renew and differentiate, and by their localization to a stem cell niche. We show here that mitotic and early meiotic germ cells are present in the adult ovary and that the zebrafish homolog of the conserved vertebrate GSC marker, nanos2, is expressed in a subset of pre-meiotic oogonia in the adult gonad. We propose that these nanos2+ cells are GSCs. Importantly, we find that mitotic, nanos2+, and early meiotic germ cells localize to the germinal zone, thus identifying this region as the probable ovarian GSC niche in zebrafish. nanos3, which encodes a conserved RNA-binding protein, is known to be required for the continued production of oocytes in the zebrafish. Although mammalian homologs of nanos3 are expressed in early spermatogonia, no study has defined the role of nanos3 in the regulation of vertebrate GSCs. Here we demonstrate that nanos3 function is required for the maintenance of GSCs, but not for their specification, and propose that nanos2 and nanos3 are partially redundant in this role.

KW - Germline stem cells

KW - Nanos2

KW - Nanos3

KW - Ovary

KW - Stem cell niche

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=84872952201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872952201&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2012.12.003

DO - 10.1016/j.ydbio.2012.12.003

M3 - Article

VL - 374

SP - 308

EP - 318

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 2

ER -