Nanoparticles for live cell microscopy: A surface-enhanced Raman scattering perspective

Maria Navas-Moreno, Majid Mehrpouyan, Tatyana Chernenko, Demet Candas, Ming Fan, Jian-Jian Li, Ming Yan, James W Chan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Surface enhanced Raman scattering (SERS) nanoparticles are an attractive alternative to fluorescent probes for biological labeling because of their photostability and multiplexing capabilities. However, nanoparticle size, shape, and surface properties are known to affect nanoparticle-cell interactions. Other issues such as the formation of a protein corona and antibody multivalency interfere with the labeling properties of nanoparticle-antibody conjugates. Hence, it is important to consider these aspects in order to validate such conjugates for live cell imaging applications. Using SERS nanoparticles that target HER2 and CD44 in breast cancer cells, we demonstrate labeling of fixed cells with high specificity that correlates well with fluorescent labels. However, when labeling live cells to monitor surface biomarker expression and dynamics, the nanoparticles are rapidly uptaken by the cells and become compartmentalized into different cellular regions. This behavior is in stark contrast to that of fluorescent antibody conjugates. This study highlights the impact of nanoparticle internalization and trafficking on the ability to use SERS nanoparticle-antibody conjugates to monitor cell dynamics.

Original languageEnglish (US)
Article number4066
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Raman scattering
Microscopic examination
Nanoparticles
Labeling
Antibodies
Biomarkers
Fluorescent Dyes
Multiplexing
Surface properties
Labels
Cells
Imaging techniques

ASJC Scopus subject areas

  • General

Cite this

Nanoparticles for live cell microscopy : A surface-enhanced Raman scattering perspective. / Navas-Moreno, Maria; Mehrpouyan, Majid; Chernenko, Tatyana; Candas, Demet; Fan, Ming; Li, Jian-Jian; Yan, Ming; Chan, James W.

In: Scientific Reports, Vol. 7, No. 1, 4066, 01.12.2017.

Research output: Contribution to journalArticle

Navas-Moreno, Maria ; Mehrpouyan, Majid ; Chernenko, Tatyana ; Candas, Demet ; Fan, Ming ; Li, Jian-Jian ; Yan, Ming ; Chan, James W. / Nanoparticles for live cell microscopy : A surface-enhanced Raman scattering perspective. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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