Nanoliquid chromatography-mass spectrometry of oligosaccharides employing graphitized carbon chromatography on microchip with a high-accuracy mass analyzer

Milady Niñonuevo, Hyunjoo An, Hongfeng Yin, Kevin Killeen, Rudi Grimm, Robert Ward, Bruce German, Carlito B Lebrilla

Research output: Contribution to journalArticle

118 Scopus citations

Abstract

The nanoLC separations of oligosaccharides using microchip-based columns are described. Mixtures of alditols from mucins and human milk are separated on graphitized carbon. The nanoLC-MS device showed high mass accuracy for the oligosaccharides ranging between 1 and 6 ppm on routine analyses. The high mass accuracy readily allowed identification of oligosaccharide peaks and the determination of their compositions. High retention time reproducibility was exhibited by the microchip LC. Little variation was observed for standard sample either alone or in a complex heterogeneous mixture. The nanoLC-MS exhibits excellent capabilities in profiling mixtures of oligosaccharides.

Original languageEnglish (US)
Pages (from-to)3641-3649
Number of pages9
JournalElectrophoresis
Volume26
Issue number19
DOIs
StatePublished - Oct 2005

Keywords

  • Mass spectrometry
  • Microfluidics
  • Miniaturization
  • Nanoliquid chromatography
  • Oligosaccharides

ASJC Scopus subject areas

  • Clinical Biochemistry

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