Nanoelectropulse intracellular perturbation and electropermeabilization technology: Phospholipid translocation, calcium bursts, chromatin rearrangement, cardiomyocyte activation, and tumor cell sensitivity

P. Thomas Vernier, Yinghua Sun, Jingjing Wang, Mya Mya Thu, Edward Garon, Miguel Valderrabano, Laura Marcu, H. Phillip Koeffler, Martin A. Gundersen

Research output: Chapter in Book/Report/Conference proceedingConference contribution

20 Citations (Scopus)

Abstract

Nanosecond, megavolt-per-meter pulsed electric fields scramble the asymmetric arrangement of phospholipids in the plasma membrane, release intracellular calcium, trigger cardiomyocyte activity, and induce apoptosis in mammalian cancer cells, without the permeabilizing effects associated with longer, lower-field pulses. Dose dependencies with respect to pulse width, amplitude, and repetition rate, and total pulse count are observed for all of these phenomena. Sensitivities vary among cell types; cells of lymphoid origin growing in suspension are more susceptible to nanoelectropulse exposure than solid tumor lines. Simple electrical models of the cell are useful for first-order explanations, but more sophisticated treatments will be required for analysis and prediction at both biomolecular and tissue levels.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages5850-5853
Number of pages4
Volume7 VOLS
StatePublished - 2005
Event2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005 - Shanghai, China
Duration: Sep 1 2005Sep 4 2005

Other

Other2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005
CountryChina
CityShanghai
Period9/1/059/4/05

Fingerprint

Phospholipids
Cell death
Cell membranes
Chromatin
Tumors
Calcium
Suspensions
Chemical activation
Cells
Electric fields
Tissue
Apoptosis

Keywords

  • Bioelectric phenomena
  • Biomembranes
  • Cancer
  • Cardiac synchronization
  • Electropermeabilization
  • Intracellular calcium
  • Nanosecond megavolt-per-meter electric pulses
  • Tumor

ASJC Scopus subject areas

  • Bioengineering

Cite this

Vernier, P. T., Sun, Y., Wang, J., Thu, M. M., Garon, E., Valderrabano, M., ... Gundersen, M. A. (2005). Nanoelectropulse intracellular perturbation and electropermeabilization technology: Phospholipid translocation, calcium bursts, chromatin rearrangement, cardiomyocyte activation, and tumor cell sensitivity. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 7 VOLS, pp. 5850-5853). [1615820]

Nanoelectropulse intracellular perturbation and electropermeabilization technology : Phospholipid translocation, calcium bursts, chromatin rearrangement, cardiomyocyte activation, and tumor cell sensitivity. / Vernier, P. Thomas; Sun, Yinghua; Wang, Jingjing; Thu, Mya Mya; Garon, Edward; Valderrabano, Miguel; Marcu, Laura; Koeffler, H. Phillip; Gundersen, Martin A.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 7 VOLS 2005. p. 5850-5853 1615820.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Vernier, PT, Sun, Y, Wang, J, Thu, MM, Garon, E, Valderrabano, M, Marcu, L, Koeffler, HP & Gundersen, MA 2005, Nanoelectropulse intracellular perturbation and electropermeabilization technology: Phospholipid translocation, calcium bursts, chromatin rearrangement, cardiomyocyte activation, and tumor cell sensitivity. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 7 VOLS, 1615820, pp. 5850-5853, 2005 27th Annual International Conference of the Engineering in Medicine and Biology Society, IEEE-EMBS 2005, Shanghai, China, 9/1/05.
Vernier PT, Sun Y, Wang J, Thu MM, Garon E, Valderrabano M et al. Nanoelectropulse intracellular perturbation and electropermeabilization technology: Phospholipid translocation, calcium bursts, chromatin rearrangement, cardiomyocyte activation, and tumor cell sensitivity. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 7 VOLS. 2005. p. 5850-5853. 1615820
Vernier, P. Thomas ; Sun, Yinghua ; Wang, Jingjing ; Thu, Mya Mya ; Garon, Edward ; Valderrabano, Miguel ; Marcu, Laura ; Koeffler, H. Phillip ; Gundersen, Martin A. / Nanoelectropulse intracellular perturbation and electropermeabilization technology : Phospholipid translocation, calcium bursts, chromatin rearrangement, cardiomyocyte activation, and tumor cell sensitivity. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 7 VOLS 2005. pp. 5850-5853
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