Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. I. Membrane separation from the cytoskeleton

Evan Evans, Volkmar Heinrich, Andrew Leung, Koji Kinoshita

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

We have used a biomembrane force probe decorated with P-selectin to form point attachments with PSGL-1 receptors on a human neutrophil (PMN) in a calcium-containing medium and then to quantify the forces experienced by the attachment during retraction of the PMN at fixed speed. From first touch to final detachment, the typical force history exhibited the following sequence of events: i), an initial linear-elastic displacement of the PMN surface, ii), an abrupt crossover to viscoplastic flow that signaled membrane separation from the interior cytoskeleton and the beginning of a membrane tether, and iii), the final detachment from the probe tip by usually one precipitous step of P-selectin:PSGL-1 dissociation. In this first article I, we focus on the initial elastic response and its termination by membrane separation from the cytoskeleton, initiating tether formation. Quantifying membrane unbinding forces for rates of loading (force/time) in the elastic regime from 240 pN/s to 38,000 pN/s, we discovered that the force distributions agreed well with the theory for kinetically limited failure of a weak bond. The kinetic rate for membrane unbinding was found to increase as an exponential function of the pulling force, characterized by an e-fold scale in force of ∼17 pN and a preexponential factor, or apparent unstressed off rate, of ∼1/s. The rheological properties of tether growth subsequent to the membrane unbinding events are presented in a companion article II.

Original languageEnglish (US)
Pages (from-to)2288-2298
Number of pages11
JournalBiophysical Journal
Volume88
Issue number3
DOIs
StatePublished - Mar 2005
Externally publishedYes

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P-Selectin
Cytoskeleton
Leukocytes
Membranes
Touch
Neutrophils
History
Calcium
Growth

ASJC Scopus subject areas

  • Biophysics

Cite this

Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. I. Membrane separation from the cytoskeleton. / Evans, Evan; Heinrich, Volkmar; Leung, Andrew; Kinoshita, Koji.

In: Biophysical Journal, Vol. 88, No. 3, 03.2005, p. 2288-2298.

Research output: Contribution to journalArticle

Evans, Evan ; Heinrich, Volkmar ; Leung, Andrew ; Kinoshita, Koji. / Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. I. Membrane separation from the cytoskeleton. In: Biophysical Journal. 2005 ; Vol. 88, No. 3. pp. 2288-2298.
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