Na,K-ATPase expression in normal and failing human left ventricle

P. D. Allen, T. A. Schmidt, J. D. Marsh, K. Kjeldsen

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The expression of the Na,K-ATPase was studied in both normal and failing human myocardium which was collected within 5 min of cardiac explantation in preparation for orthotopic transplantation or at the time of organ harvest. Abundance of mRNA for all three catalytic α subunits of the Na,K-ATPase was analyzed in samples from patients with end-stage heart failure due to either ischemic or dilated cardiomyopathy, as well as from normal controls. Vanadate facilitated 3H-oubain binding before and after a Digibind wash was analyzed on tissue from a subset of these patients. mRNA analysis demonstrated that all three catalytic Na,K-ATPase α subunits were expressed in human heart and that there was no evidence for change in relative expression or abundance induced by disease. The specific digitalis receptor concentration was 760 ± 58 and 614 ± 47 pmol/g wet weight in the samples from normal and failing hearts, respectively (p = NS). From these studies it can be concluded that, whereas there is a tendency for a decrease in the number of oubain receptors in heart failure, there is no significant alteration in the expression of Na,K-ATPase message or protein caused by chronic heart failure.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalBasic Research in Cardiology
Volume87
Issue numberSUPPL. 1
DOIs
StatePublished - 1992
Externally publishedYes

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Keywords

  • 3H-oubain binding
  • Heart
  • Heart failure
  • mRNA
  • Myocardium
  • Na,K-ATPase

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Allen, P. D., Schmidt, T. A., Marsh, J. D., & Kjeldsen, K. (1992). Na,K-ATPase expression in normal and failing human left ventricle. Basic Research in Cardiology, 87(SUPPL. 1), 87-94. https://doi.org/10.1007/BF00795393