Na,K-ATPase expression in normal and failing human left ventricle

P. D. Allen, T. A. Schmidt, J. D. Marsh, K. Kjeldsen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The expression of the Na,K-ATPase was studied in both normal and failing human myocardium which was collected within 5 min of cardiac explantation in preparation for orthotopic transplantation or at the time of organ harvest. Abundance of mRNA for all three catalytic α subunits of the Na,K-ATPase was analyzed in samples from patients with end-stage heart failure due to either ischemic or dilated cardiomyopathy, as well as from normal controls. Vanadate facilitated 3H-oubain binding before and after a Digibind wash was analyzed on tissue from a subset of these patients. mRNA analysis demonstrated that all three catalytic Na,K-ATPase α subunits were expressed in human heart and that there was no evidence for change in relative expression or abundance induced by disease. The specific digitalis receptor concentration was 760 ± 58 and 614 ± 47 pmol/g wet weight in the samples from normal and failing hearts, respectively (p = NS). From these studies it can be concluded that, whereas there is a tendency for a decrease in the number of oubain receptors in heart failure, there is no significant alteration in the expression of Na,K-ATPase message or protein caused by chronic heart failure.

Original languageEnglish (US)
Pages (from-to)87-94
Number of pages8
JournalBasic Research in Cardiology
Volume87
Issue numberSUPPL. 1
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Heart Ventricles
Heart Failure
Messenger RNA
Vanadates
Dilated Cardiomyopathy
Catalytic Domain
Myocardium
Transplantation
Weights and Measures
sodium-translocating ATPase
Proteins

Keywords

  • 3H-oubain binding
  • Heart
  • Heart failure
  • mRNA
  • Myocardium
  • Na,K-ATPase

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Allen, P. D., Schmidt, T. A., Marsh, J. D., & Kjeldsen, K. (1992). Na,K-ATPase expression in normal and failing human left ventricle. Basic Research in Cardiology, 87(SUPPL. 1), 87-94. https://doi.org/10.1007/BF00795393

Na,K-ATPase expression in normal and failing human left ventricle. / Allen, P. D.; Schmidt, T. A.; Marsh, J. D.; Kjeldsen, K.

In: Basic Research in Cardiology, Vol. 87, No. SUPPL. 1, 1992, p. 87-94.

Research output: Contribution to journalArticle

Allen, PD, Schmidt, TA, Marsh, JD & Kjeldsen, K 1992, 'Na,K-ATPase expression in normal and failing human left ventricle', Basic Research in Cardiology, vol. 87, no. SUPPL. 1, pp. 87-94. https://doi.org/10.1007/BF00795393
Allen, P. D. ; Schmidt, T. A. ; Marsh, J. D. ; Kjeldsen, K. / Na,K-ATPase expression in normal and failing human left ventricle. In: Basic Research in Cardiology. 1992 ; Vol. 87, No. SUPPL. 1. pp. 87-94.
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