Naive CD4+ T Cell Frequency Varies for Different Epitopes and Predicts Repertoire Diversity and Response Magnitude

James J. Moon, H. Hamlet Chu, Marion Pepper, Stephen J Mcsorley, Stephen C. Jameson, Ross M Kedl, Marc K. Jenkins

Research output: Contribution to journalArticle

596 Citations (Scopus)

Abstract

Cell-mediated immunity stems from the proliferation of naive T lymphocytes expressing T cell antigen receptors (TCRs) specific for foreign peptides bound to host major histocompatibility complex (MHC) molecules. Because of the tremendous diversity of the T cell repertoire, naive T cells specific for any one peptide:MHC complex (pMHC) are extremely rare. Thus, it is not known how many naive T cells of any given pMHC specificity exist in the body or how that number influences the immune response. By using soluble pMHC class II (pMHCII) tetramers and magnetic bead enrichment, we found that three different pMHCII-specific naive CD4+ T cell populations vary in frequency from 20 to 200 cells per mouse. Moreover, naive population size predicted the size and TCR diversity of the primary CD4+ T cell response after immunization with relevant peptide. Thus, variation in naive T cell frequencies can explain why some peptides are stronger immunogens than others.

Original languageEnglish (US)
Pages (from-to)203-213
Number of pages11
JournalImmunity
Volume27
Issue number2
DOIs
StatePublished - Aug 24 2007
Externally publishedYes

Fingerprint

Epitopes
T-Lymphocytes
Peptides
T-Cell Antigen Receptor
Major Histocompatibility Complex
Population Density
Cellular Immunity
Immunization
Population

Keywords

  • CELLIMMUNO

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

Cite this

Naive CD4+ T Cell Frequency Varies for Different Epitopes and Predicts Repertoire Diversity and Response Magnitude. / Moon, James J.; Chu, H. Hamlet; Pepper, Marion; Mcsorley, Stephen J; Jameson, Stephen C.; Kedl, Ross M; Jenkins, Marc K.

In: Immunity, Vol. 27, No. 2, 24.08.2007, p. 203-213.

Research output: Contribution to journalArticle

Moon, James J. ; Chu, H. Hamlet ; Pepper, Marion ; Mcsorley, Stephen J ; Jameson, Stephen C. ; Kedl, Ross M ; Jenkins, Marc K. / Naive CD4+ T Cell Frequency Varies for Different Epitopes and Predicts Repertoire Diversity and Response Magnitude. In: Immunity. 2007 ; Vol. 27, No. 2. pp. 203-213.
@article{b0478abb1a8c48fab61cb6dfc9de6857,
title = "Naive CD4+ T Cell Frequency Varies for Different Epitopes and Predicts Repertoire Diversity and Response Magnitude",
abstract = "Cell-mediated immunity stems from the proliferation of naive T lymphocytes expressing T cell antigen receptors (TCRs) specific for foreign peptides bound to host major histocompatibility complex (MHC) molecules. Because of the tremendous diversity of the T cell repertoire, naive T cells specific for any one peptide:MHC complex (pMHC) are extremely rare. Thus, it is not known how many naive T cells of any given pMHC specificity exist in the body or how that number influences the immune response. By using soluble pMHC class II (pMHCII) tetramers and magnetic bead enrichment, we found that three different pMHCII-specific naive CD4+ T cell populations vary in frequency from 20 to 200 cells per mouse. Moreover, naive population size predicted the size and TCR diversity of the primary CD4+ T cell response after immunization with relevant peptide. Thus, variation in naive T cell frequencies can explain why some peptides are stronger immunogens than others.",
keywords = "CELLIMMUNO",
author = "Moon, {James J.} and Chu, {H. Hamlet} and Marion Pepper and Mcsorley, {Stephen J} and Jameson, {Stephen C.} and Ross M Kedl and Jenkins, {Marc K.}",
year = "2007",
month = "8",
day = "24",
doi = "10.1016/j.immuni.2007.07.007",
language = "English (US)",
volume = "27",
pages = "203--213",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Naive CD4+ T Cell Frequency Varies for Different Epitopes and Predicts Repertoire Diversity and Response Magnitude

AU - Moon, James J.

AU - Chu, H. Hamlet

AU - Pepper, Marion

AU - Mcsorley, Stephen J

AU - Jameson, Stephen C.

AU - Kedl, Ross M

AU - Jenkins, Marc K.

PY - 2007/8/24

Y1 - 2007/8/24

N2 - Cell-mediated immunity stems from the proliferation of naive T lymphocytes expressing T cell antigen receptors (TCRs) specific for foreign peptides bound to host major histocompatibility complex (MHC) molecules. Because of the tremendous diversity of the T cell repertoire, naive T cells specific for any one peptide:MHC complex (pMHC) are extremely rare. Thus, it is not known how many naive T cells of any given pMHC specificity exist in the body or how that number influences the immune response. By using soluble pMHC class II (pMHCII) tetramers and magnetic bead enrichment, we found that three different pMHCII-specific naive CD4+ T cell populations vary in frequency from 20 to 200 cells per mouse. Moreover, naive population size predicted the size and TCR diversity of the primary CD4+ T cell response after immunization with relevant peptide. Thus, variation in naive T cell frequencies can explain why some peptides are stronger immunogens than others.

AB - Cell-mediated immunity stems from the proliferation of naive T lymphocytes expressing T cell antigen receptors (TCRs) specific for foreign peptides bound to host major histocompatibility complex (MHC) molecules. Because of the tremendous diversity of the T cell repertoire, naive T cells specific for any one peptide:MHC complex (pMHC) are extremely rare. Thus, it is not known how many naive T cells of any given pMHC specificity exist in the body or how that number influences the immune response. By using soluble pMHC class II (pMHCII) tetramers and magnetic bead enrichment, we found that three different pMHCII-specific naive CD4+ T cell populations vary in frequency from 20 to 200 cells per mouse. Moreover, naive population size predicted the size and TCR diversity of the primary CD4+ T cell response after immunization with relevant peptide. Thus, variation in naive T cell frequencies can explain why some peptides are stronger immunogens than others.

KW - CELLIMMUNO

UR - http://www.scopus.com/inward/record.url?scp=34548038390&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548038390&partnerID=8YFLogxK

U2 - 10.1016/j.immuni.2007.07.007

DO - 10.1016/j.immuni.2007.07.007

M3 - Article

C2 - 17707129

AN - SCOPUS:34548038390

VL - 27

SP - 203

EP - 213

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 2

ER -