Na: Ca stoichiometry and cytosolic Ca-dependent activation of NCX in intact cardiomyocytes

Donald M Bers; Kenneth S Ginsburg

doi:10.1196/annals.1387.060

Na: Ca stoichiometry and cytosolic Ca-dependent activation of NCX in intact cardiomyocytes

Donald M Bers, Kenneth S Ginsburg

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

33 Scopus citations

Abstract

We are studying both Na:Ca exchange stoichiometry and cytosolic [Ca] ([Ca]_i)-dependent regulation of Na-Ca exchange (NCX) in intact rabbit ventricular myocytes. Analysis of NCX fluxes in subcellular systems strongly supports a dominant 3Na:1Ca exchange, and our measurements in intact cells confirm this. However, in intact native cells, local ion gradients and other factors complicate the process of inferring stoichiometry. From a functional viewpoint, NCX stoichiometry is near 3:1 but is affected by ion accumulation/depletion as well as non-NCX fluxes. We and others have viewed [Ca]_i-dependent NCX regulation as a static process (dependent on instantaneous local [Ca]_i). However, evidence from subcellular and expression systems shows the process to be dynamic, and our observations confirm this to be the case in intact cardiac cells as well.

Original language	English (US)
Title of host publication	Annals of the New York Academy of Sciences
Pages	326-338
Number of pages	13
Volume	1099
DOIs	https://doi.org/10.1196/annals.1387.060
State	Published - Mar 2007
Externally published	Yes

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1099
ISSN (Print)	00778923
ISSN (Electronic)	17496632

Keywords

Ca-dependent regulation
Calcium
Na-Ca exchange
Na:Ca stoichiometry
Sodium

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.1196/annals.1387.060

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title = "Na: Ca stoichiometry and cytosolic Ca-dependent activation of NCX in intact cardiomyocytes",

abstract = "We are studying both Na:Ca exchange stoichiometry and cytosolic [Ca] ([Ca]i)-dependent regulation of Na-Ca exchange (NCX) in intact rabbit ventricular myocytes. Analysis of NCX fluxes in subcellular systems strongly supports a dominant 3Na:1Ca exchange, and our measurements in intact cells confirm this. However, in intact native cells, local ion gradients and other factors complicate the process of inferring stoichiometry. From a functional viewpoint, NCX stoichiometry is near 3:1 but is affected by ion accumulation/depletion as well as non-NCX fluxes. We and others have viewed [Ca]i-dependent NCX regulation as a static process (dependent on instantaneous local [Ca]i). However, evidence from subcellular and expression systems shows the process to be dynamic, and our observations confirm this to be the case in intact cardiac cells as well.",

keywords = "Ca-dependent regulation, Calcium, Na-Ca exchange, Na:Ca stoichiometry, Sodium",

author = "Bers, {Donald M} and Ginsburg, {Kenneth S}",

year = "2007",

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AB - We are studying both Na:Ca exchange stoichiometry and cytosolic [Ca] ([Ca]i)-dependent regulation of Na-Ca exchange (NCX) in intact rabbit ventricular myocytes. Analysis of NCX fluxes in subcellular systems strongly supports a dominant 3Na:1Ca exchange, and our measurements in intact cells confirm this. However, in intact native cells, local ion gradients and other factors complicate the process of inferring stoichiometry. From a functional viewpoint, NCX stoichiometry is near 3:1 but is affected by ion accumulation/depletion as well as non-NCX fluxes. We and others have viewed [Ca]i-dependent NCX regulation as a static process (dependent on instantaneous local [Ca]i). However, evidence from subcellular and expression systems shows the process to be dynamic, and our observations confirm this to be the case in intact cardiac cells as well.

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