Na: Ca stoichiometry and cytosolic Ca-dependent activation of NCX in intact cardiomyocytes

Donald M Bers, Kenneth S Ginsburg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

30 Scopus citations

Abstract

We are studying both Na:Ca exchange stoichiometry and cytosolic [Ca] ([Ca]i)-dependent regulation of Na-Ca exchange (NCX) in intact rabbit ventricular myocytes. Analysis of NCX fluxes in subcellular systems strongly supports a dominant 3Na:1Ca exchange, and our measurements in intact cells confirm this. However, in intact native cells, local ion gradients and other factors complicate the process of inferring stoichiometry. From a functional viewpoint, NCX stoichiometry is near 3:1 but is affected by ion accumulation/depletion as well as non-NCX fluxes. We and others have viewed [Ca]i-dependent NCX regulation as a static process (dependent on instantaneous local [Ca]i). However, evidence from subcellular and expression systems shows the process to be dynamic, and our observations confirm this to be the case in intact cardiac cells as well.

Original languageEnglish (US)
Title of host publicationAnnals of the New York Academy of Sciences
Pages326-338
Number of pages13
Volume1099
DOIs
StatePublished - Mar 2007
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1099
ISSN (Print)00778923
ISSN (Electronic)17496632

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Keywords

  • Ca-dependent regulation
  • Calcium
  • Na-Ca exchange
  • Na:Ca stoichiometry
  • Sodium

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Bers, D. M., & Ginsburg, K. S. (2007). Na: Ca stoichiometry and cytosolic Ca-dependent activation of NCX in intact cardiomyocytes. In Annals of the New York Academy of Sciences (Vol. 1099, pp. 326-338). (Annals of the New York Academy of Sciences; Vol. 1099). https://doi.org/10.1196/annals.1387.060