N-terminal hemagglutinin tag renders lysine-deficient APOBEC3G resistant to HIV-1 vif-induced degradation by reduced polyubiquitination

Yudi Wang, Qiujia Shao, Xianghui Yu, Wei Kong, James Hildreth, Bindong Liu

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

APOBEC3G, a potent HIV-1 host restriction factor, is overcome by HIV-1 viral infectivity factor (Vif), which induces its polyubiquitination and proteasomal degradation. Here we show that lysine-deficient APOBEC3G with an N-terminal hemagglutinin (HA) tag fusion (HA-A3G20K/R) was resistant to HIV-1 Vif-induced proteasomal degradation. HA-A3G20K/R molecules were packaged into wild-type HIV-1 particles, and HA-A3G20K/R drastically decreased wild-type HIV-1 reverse transcription products and infectivity. We also showed that the N terminus of A3G was a target of polyubiquitination induced by HIV-1 Vif. Thus, fusion of the HA tag to the N terminus of A3G20K/R reduced its polyubiquitination, the likely mechanism for the resistance of this protein to HIV-1 Vif-induced proteasomal degradation. Finding such ways to induce resistance of A3G to Vif may provide new approaches to anti-HIV/AIDS therapy.

Original languageEnglish (US)
Pages (from-to)4510-4519
Number of pages10
JournalJournal of Virology
Volume85
Issue number9
DOIs
StatePublished - May 2011
Externally publishedYes

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Hemagglutinins
hemagglutinins
Human immunodeficiency virus 1
Lysine
HIV-1
lysine
pathogenicity
degradation
reverse transcription
ubiquitination
resistance mechanisms
Reverse Transcription
Acquired Immunodeficiency Syndrome
HIV
therapeutics
Proteins
proteins

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

N-terminal hemagglutinin tag renders lysine-deficient APOBEC3G resistant to HIV-1 vif-induced degradation by reduced polyubiquitination. / Wang, Yudi; Shao, Qiujia; Yu, Xianghui; Kong, Wei; Hildreth, James; Liu, Bindong.

In: Journal of Virology, Vol. 85, No. 9, 05.2011, p. 4510-4519.

Research output: Contribution to journalArticle

Wang, Yudi ; Shao, Qiujia ; Yu, Xianghui ; Kong, Wei ; Hildreth, James ; Liu, Bindong. / N-terminal hemagglutinin tag renders lysine-deficient APOBEC3G resistant to HIV-1 vif-induced degradation by reduced polyubiquitination. In: Journal of Virology. 2011 ; Vol. 85, No. 9. pp. 4510-4519.
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