N-methyl-d-aspartate receptors mediate endogenous opioid release in enteric neurons after abdominal surgery

Simona Patierno, Wubanche Zellalem, Anthony Ho, Chris G. Parsons, Kevin C K Lloyd, Marcello Tonini, Catia Sternini

Research output: Contribution to journalArticle

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Abstract

Background & Aims: We tested the hypothesis that N-methyl-d-aspartate (NMDA) receptors mediate surgery-induced opioid release in enteric neurons. Methods: We used μ opioid receptor (μOR) internalization as a measure of opioid release with immunohistochemistry and confocal microscopy. μOR internalization was quantified in enteric neurons from nondenervated and denervated ileal segments of guinea pig after abdominal laparotomy with and without pretreatment with NMDA-receptor antagonists acting at different recognition sites (+)-5-methyl-10, 11-dihydro-5H-dibenzo [a, b] cyclohepten-5, 10-imine (MK-801) or (D) 2-amino-5-phosphopenoic acid (AP-5) at. 5, 1 mg/kg; 8-chloro-4-hydroxy-1-oxo-1, 2-dihydropyridazinol [4,5-]quinoline-5-oxide choline (MRZ 2/576) or 8-chloro-1, 4-dioxo-1,2,3,4-tetrahydropyridazinol [4,5-]quinoline choline salt (MRZ 2/596) at. 3, 1 mg/kg, or with an antagonist for the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, 6-cyano-7-nitroquinoxaline-2, 3-dione (1, 3 mg/kg). To determine whether NMDA stimulation induces opioid release, (1) ilea were exposed to NMDA (100 μmol/L) and D-serine (10 μmol/L) with or without the antagonist MK-801 or AP-5 (50 μmol/L); and (2) neuromuscular preparations of the ileum were stimulated electrically (20 Hz, 20 min) with or without MK-801 or AP-5 (50 μmol/L). Results: μOR endocytosis induced by abdominal laparotomy was inhibited significantly by NMDA-receptor antagonists in nondenervated and denervated ileal segments, but not by the AMPA-receptor antagonist. μOR endocytosis in neurons exposed to NMDA or electrical stimulation was prevented by NMDA-R antagonists. Conclusions: Abdominal laparotomy evokes local release of glutamate that results in endogenous opioid release through the activation of peripheral NMDA receptors. This suggests an interaction between the glutamatergic and opioid systems in response to the noxious and perhaps mechanosensory stimulation of surgery.

Original languageEnglish (US)
Pages (from-to)2009-2019
Number of pages11
JournalGastroenterology
Volume128
Issue number7
DOIs
StatePublished - Jun 2005

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Opioid Analgesics
Opioid Receptors
Aspartic Acid
Dizocilpine Maleate
Neurons
Laparotomy
AMPA Receptors
Endocytosis
Choline
Ileum
6-Cyano-7-nitroquinoxaline-2,3-dione
Acids
Imines
Confocal Microscopy
Oxides
Serine
Electric Stimulation
Glutamic Acid
Guinea Pigs
Salts

ASJC Scopus subject areas

  • Gastroenterology

Cite this

N-methyl-d-aspartate receptors mediate endogenous opioid release in enteric neurons after abdominal surgery. / Patierno, Simona; Zellalem, Wubanche; Ho, Anthony; Parsons, Chris G.; Lloyd, Kevin C K; Tonini, Marcello; Sternini, Catia.

In: Gastroenterology, Vol. 128, No. 7, 06.2005, p. 2009-2019.

Research output: Contribution to journalArticle

Patierno, Simona ; Zellalem, Wubanche ; Ho, Anthony ; Parsons, Chris G. ; Lloyd, Kevin C K ; Tonini, Marcello ; Sternini, Catia. / N-methyl-d-aspartate receptors mediate endogenous opioid release in enteric neurons after abdominal surgery. In: Gastroenterology. 2005 ; Vol. 128, No. 7. pp. 2009-2019.
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abstract = "Background & Aims: We tested the hypothesis that N-methyl-d-aspartate (NMDA) receptors mediate surgery-induced opioid release in enteric neurons. Methods: We used μ opioid receptor (μOR) internalization as a measure of opioid release with immunohistochemistry and confocal microscopy. μOR internalization was quantified in enteric neurons from nondenervated and denervated ileal segments of guinea pig after abdominal laparotomy with and without pretreatment with NMDA-receptor antagonists acting at different recognition sites (+)-5-methyl-10, 11-dihydro-5H-dibenzo [a, b] cyclohepten-5, 10-imine (MK-801) or (D) 2-amino-5-phosphopenoic acid (AP-5) at. 5, 1 mg/kg; 8-chloro-4-hydroxy-1-oxo-1, 2-dihydropyridazinol [4,5-]quinoline-5-oxide choline (MRZ 2/576) or 8-chloro-1, 4-dioxo-1,2,3,4-tetrahydropyridazinol [4,5-]quinoline choline salt (MRZ 2/596) at. 3, 1 mg/kg, or with an antagonist for the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, 6-cyano-7-nitroquinoxaline-2, 3-dione (1, 3 mg/kg). To determine whether NMDA stimulation induces opioid release, (1) ilea were exposed to NMDA (100 μmol/L) and D-serine (10 μmol/L) with or without the antagonist MK-801 or AP-5 (50 μmol/L); and (2) neuromuscular preparations of the ileum were stimulated electrically (20 Hz, 20 min) with or without MK-801 or AP-5 (50 μmol/L). Results: μOR endocytosis induced by abdominal laparotomy was inhibited significantly by NMDA-receptor antagonists in nondenervated and denervated ileal segments, but not by the AMPA-receptor antagonist. μOR endocytosis in neurons exposed to NMDA or electrical stimulation was prevented by NMDA-R antagonists. Conclusions: Abdominal laparotomy evokes local release of glutamate that results in endogenous opioid release through the activation of peripheral NMDA receptors. This suggests an interaction between the glutamatergic and opioid systems in response to the noxious and perhaps mechanosensory stimulation of surgery.",
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AU - Zellalem, Wubanche

AU - Ho, Anthony

AU - Parsons, Chris G.

AU - Lloyd, Kevin C K

AU - Tonini, Marcello

AU - Sternini, Catia

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N2 - Background & Aims: We tested the hypothesis that N-methyl-d-aspartate (NMDA) receptors mediate surgery-induced opioid release in enteric neurons. Methods: We used μ opioid receptor (μOR) internalization as a measure of opioid release with immunohistochemistry and confocal microscopy. μOR internalization was quantified in enteric neurons from nondenervated and denervated ileal segments of guinea pig after abdominal laparotomy with and without pretreatment with NMDA-receptor antagonists acting at different recognition sites (+)-5-methyl-10, 11-dihydro-5H-dibenzo [a, b] cyclohepten-5, 10-imine (MK-801) or (D) 2-amino-5-phosphopenoic acid (AP-5) at. 5, 1 mg/kg; 8-chloro-4-hydroxy-1-oxo-1, 2-dihydropyridazinol [4,5-]quinoline-5-oxide choline (MRZ 2/576) or 8-chloro-1, 4-dioxo-1,2,3,4-tetrahydropyridazinol [4,5-]quinoline choline salt (MRZ 2/596) at. 3, 1 mg/kg, or with an antagonist for the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors, 6-cyano-7-nitroquinoxaline-2, 3-dione (1, 3 mg/kg). To determine whether NMDA stimulation induces opioid release, (1) ilea were exposed to NMDA (100 μmol/L) and D-serine (10 μmol/L) with or without the antagonist MK-801 or AP-5 (50 μmol/L); and (2) neuromuscular preparations of the ileum were stimulated electrically (20 Hz, 20 min) with or without MK-801 or AP-5 (50 μmol/L). Results: μOR endocytosis induced by abdominal laparotomy was inhibited significantly by NMDA-receptor antagonists in nondenervated and denervated ileal segments, but not by the AMPA-receptor antagonist. μOR endocytosis in neurons exposed to NMDA or electrical stimulation was prevented by NMDA-R antagonists. Conclusions: Abdominal laparotomy evokes local release of glutamate that results in endogenous opioid release through the activation of peripheral NMDA receptors. This suggests an interaction between the glutamatergic and opioid systems in response to the noxious and perhaps mechanosensory stimulation of surgery.

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