Myxobolus cerebralis internal transcribed spacer 1 (ITS-1) sequences support recent spread of the parasite to North America and within Europe

Christopher M. Whipps, Mansour El-Matbouli, Ronald Hedrick, Vicki Blazer, Michael L. Kent

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Molecular approaches for resolving relationships among the Myxozoa have relied mainly on small subunit (SSU) ribosomal DNA (rDNA) sequence analysis. This region of the gene is generally used for higher phylogenetic studies, and the conservative nature of this gene may make it inadequate for intraspecific comparisons. Previous intraspecific studies of Myxobolus cerebralis based on molecular analyses reported that the sequence of SSU rDNA and the internal transcribed spacer (ITS) were highly conserved in representatives of the parasite from North America and Europe. Considering that the ITS is usually a more variable region than the SSU, we reanalyzed available sequences on GenBank and obtained sequences from other M. cerebralis representatives from the states of California and West Virginia in the USA and from Germany and Russia. With the exception of 7 base pairs, most of the sequence designated as ITS-1 in GenBank was a highly conserved portion of the rDNA near the 3-prime end of the SSU region. Nonetheless, the additional ITS-1 sequences obtained from the available geographic representatives were well conserved. It is unlikely that we would have observed virtually identical ITS-1 sequences between European and American M. cerebralis samples had it spread naturally over time, particularly when compared to the variation seen between isolates of another myxozoan (Kudoa thyrsites) that has most likely spread naturally. These data further support the hypothesis that the current distribution of M. cerebralis in North America is a result of recent introductions followed by dispersal via anthropogenic means, largely through the stocking of infected trout for sport fishing.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalDiseases of Aquatic Organisms
Volume60
Issue number2
DOIs
StatePublished - Aug 9 2004

Fingerprint

Myxobolus cerebralis
internal transcribed spacers
parasite
DNA
parasites
ribosomal DNA
sport fishing
gene
Kudoa
Myxozoa
phylogenetics
trout
Russia
genes
sequence analysis
Germany
North America
Europe
nucleotide sequences
phylogeny

Keywords

  • Internal transcribed spacer
  • Myxobolus cerebralis
  • Myxozoa
  • Ribosomal DNA
  • Whirling disease

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

Cite this

Myxobolus cerebralis internal transcribed spacer 1 (ITS-1) sequences support recent spread of the parasite to North America and within Europe. / Whipps, Christopher M.; El-Matbouli, Mansour; Hedrick, Ronald; Blazer, Vicki; Kent, Michael L.

In: Diseases of Aquatic Organisms, Vol. 60, No. 2, 09.08.2004, p. 105-108.

Research output: Contribution to journalArticle

Whipps, Christopher M. ; El-Matbouli, Mansour ; Hedrick, Ronald ; Blazer, Vicki ; Kent, Michael L. / Myxobolus cerebralis internal transcribed spacer 1 (ITS-1) sequences support recent spread of the parasite to North America and within Europe. In: Diseases of Aquatic Organisms. 2004 ; Vol. 60, No. 2. pp. 105-108.
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