TY - JOUR
T1 - Myxobolus cerebralis
T2 - Identification of a cathepsin Z-like protease gene (MyxCP-1) expressed during parasite development in rainbow trout, Oncorhynchus mykiss
AU - Kelley, Garry O.
AU - Adkison, Mark A.
AU - Leutenegger, Christian M.
AU - Hedrick, Ronald
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Cysteine proteases are essential to the biological function of parasitic organisms and are gaining interest as the target of chemotherapeutics aimed at their control. We report a cysteine protease gene from the myxozoan Myxobolus cerebralis, the cause of whirling disease in salmonid fish. The mature gene (MyxCP-1) encodes a 248-amino acid polypeptide that includes catalytic residues similar to the papain family of cysteine proteases. MyxCP-1 features a propeptide region and sequence insertions that are characteristics of cathepsin Z proteases. Phylogenetic comparisons of M. cerebralis to other eukaryotes based on full-length cathepsin-like genes show that MyxCP-1 is the earliest lineage in the cathepsin Z group and separated from cathepsin L, B, and C-like proteases. Using TaqMan PCR differential levels of transcription of the cathepsin Z-like protease were found in earlier and later developmental stages of the parasite in experimentally infected rainbow trout (Oncorhynchus mykiss).
AB - Cysteine proteases are essential to the biological function of parasitic organisms and are gaining interest as the target of chemotherapeutics aimed at their control. We report a cysteine protease gene from the myxozoan Myxobolus cerebralis, the cause of whirling disease in salmonid fish. The mature gene (MyxCP-1) encodes a 248-amino acid polypeptide that includes catalytic residues similar to the papain family of cysteine proteases. MyxCP-1 features a propeptide region and sequence insertions that are characteristics of cathepsin Z proteases. Phylogenetic comparisons of M. cerebralis to other eukaryotes based on full-length cathepsin-like genes show that MyxCP-1 is the earliest lineage in the cathepsin Z group and separated from cathepsin L, B, and C-like proteases. Using TaqMan PCR differential levels of transcription of the cathepsin Z-like protease were found in earlier and later developmental stages of the parasite in experimentally infected rainbow trout (Oncorhynchus mykiss).
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U2 - 10.1016/j.exppara.2003.12.004
DO - 10.1016/j.exppara.2003.12.004
M3 - Article
C2 - 14990313
AN - SCOPUS:1342265418
VL - 105
SP - 201
EP - 210
JO - Experimental Parasitology
JF - Experimental Parasitology
SN - 0014-4894
IS - 3-4
ER -