Myosin IIA dependent retrograde flow drives 3D cell migration

Wenting Shih, Soichiro Yamada

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Epithelial cell migration is an essential part of embryogenesis and tissue regeneration, yet their migration is least understood. Using our three-dimensional (3D) motility analysis, migrating epithelial cells formed an atypical polarized cell shape with the nucleus leading the cell front and a contractile cell rear. Migrating epithelial cells exerted traction forces to deform both the anterior and posterior extracellular matrix toward the cell body. The cell leading edge exhibited a myosin II-dependent retrograde flow with the magnitude and direction consistent with surrounding network deformation. Interestingly, on a two-dimensional substrate, myosin IIA-deficient cells migrated faster than wild-type cells, but in a 3D gel, these myosin IIA-deficient cells were unpolarized and immobile. In contrast, the migration rates of myosin IIB-deficient cells were similar to wild-type cells. Therefore, myosin IIA, not myosin IIB, is required for 3D epithelial cell migration.

Original languageEnglish (US)
JournalBiophysical Journal
Volume98
Issue number8
DOIs
StatePublished - Apr 21 2010

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Nonmuscle Myosin Type IIA
Cell Movement
Nonmuscle Myosin Type IIB
Epithelial Cells
Cell Nucleus Shape
Myosin Type II
Traction
Embryonic Development
Extracellular Matrix
Regeneration
Gels

ASJC Scopus subject areas

  • Biophysics

Cite this

Myosin IIA dependent retrograde flow drives 3D cell migration. / Shih, Wenting; Yamada, Soichiro.

In: Biophysical Journal, Vol. 98, No. 8, 21.04.2010.

Research output: Contribution to journalArticle

Shih, Wenting ; Yamada, Soichiro. / Myosin IIA dependent retrograde flow drives 3D cell migration. In: Biophysical Journal. 2010 ; Vol. 98, No. 8.
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