Abstract
Epithelial cell migration is an essential part of embryogenesis and tissue regeneration, yet their migration is least understood. Using our three-dimensional (3D) motility analysis, migrating epithelial cells formed an atypical polarized cell shape with the nucleus leading the cell front and a contractile cell rear. Migrating epithelial cells exerted traction forces to deform both the anterior and posterior extracellular matrix toward the cell body. The cell leading edge exhibited a myosin II-dependent retrograde flow with the magnitude and direction consistent with surrounding network deformation. Interestingly, on a two-dimensional substrate, myosin IIA-deficient cells migrated faster than wild-type cells, but in a 3D gel, these myosin IIA-deficient cells were unpolarized and immobile. In contrast, the migration rates of myosin IIB-deficient cells were similar to wild-type cells. Therefore, myosin IIA, not myosin IIB, is required for 3D epithelial cell migration.
| Original language | English (US) |
|---|---|
| Journal | Biophysical Journal |
| Volume | 98 |
| Issue number | 8 |
| DOIs | |
| State | Published - Apr 21 2010 |
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ASJC Scopus subject areas
- Biophysics
Cite this
Myosin IIA dependent retrograde flow drives 3D cell migration. / Shih, Wenting; Yamada, Soichiro.
In: Biophysical Journal, Vol. 98, No. 8, 21.04.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Myosin IIA dependent retrograde flow drives 3D cell migration
AU - Shih, Wenting
AU - Yamada, Soichiro
PY - 2010/4/21
Y1 - 2010/4/21
N2 - Epithelial cell migration is an essential part of embryogenesis and tissue regeneration, yet their migration is least understood. Using our three-dimensional (3D) motility analysis, migrating epithelial cells formed an atypical polarized cell shape with the nucleus leading the cell front and a contractile cell rear. Migrating epithelial cells exerted traction forces to deform both the anterior and posterior extracellular matrix toward the cell body. The cell leading edge exhibited a myosin II-dependent retrograde flow with the magnitude and direction consistent with surrounding network deformation. Interestingly, on a two-dimensional substrate, myosin IIA-deficient cells migrated faster than wild-type cells, but in a 3D gel, these myosin IIA-deficient cells were unpolarized and immobile. In contrast, the migration rates of myosin IIB-deficient cells were similar to wild-type cells. Therefore, myosin IIA, not myosin IIB, is required for 3D epithelial cell migration.
AB - Epithelial cell migration is an essential part of embryogenesis and tissue regeneration, yet their migration is least understood. Using our three-dimensional (3D) motility analysis, migrating epithelial cells formed an atypical polarized cell shape with the nucleus leading the cell front and a contractile cell rear. Migrating epithelial cells exerted traction forces to deform both the anterior and posterior extracellular matrix toward the cell body. The cell leading edge exhibited a myosin II-dependent retrograde flow with the magnitude and direction consistent with surrounding network deformation. Interestingly, on a two-dimensional substrate, myosin IIA-deficient cells migrated faster than wild-type cells, but in a 3D gel, these myosin IIA-deficient cells were unpolarized and immobile. In contrast, the migration rates of myosin IIB-deficient cells were similar to wild-type cells. Therefore, myosin IIA, not myosin IIB, is required for 3D epithelial cell migration.
UR - http://www.scopus.com/inward/record.url?scp=77951647447&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951647447&partnerID=8YFLogxK
U2 - 10.1016/j.bpj.2010.02.028
DO - 10.1016/j.bpj.2010.02.028
M3 - Article
C2 - 20409454
AN - SCOPUS:77951647447
VL - 98
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 8
ER -