Myosin IIA deficient cells migrate efficiently despite reduced traction forces at cell periphery

Melissa H. Jorrisch, Wenting Shih, Soichiro Yamada

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Cell motility is a cornerstone of embryogenesis, tissue remodeling and repair, and cancer cell invasion. It is generally thought that migrating cells grab and exert traction force onto the extracellular matrix in order to pull the cell body forward. While previous studies have shown that myosin II deficient cells migrate efficiently, whether these cells exert traction forces during cell migration in the absence of the major contractile machinery is currently unknown. Using an array of micronsized pillars as a force sensor and shRNA specific to eachmyosin II isoform (A and B), we analyzed how myosin IIA and IIB individually regulate cell migration and traction force generation. Myosin IIA and IIB localized preferentially to the leading edge where traction force was greatest, and the trailing edge, respectively. When individual myosin II isoforms were depleted by shRNA, myosin IIA deficient cells lost actin stress fibers and focal adhesions, whereas myosin IIB deficient cells maintained similar actin organization and focal adhesions as wild-type cells. Interestingly, myosin IIA deficient cells migrated faster than wild-type ormyosin IIB deficient cells on both a rigid surface and a pillar array, yet myosin IIA deficient cells exerted significantly less traction force at the leading edge than wildtype or myosin IIB deficient cells. These results suggest that, in the absence of myosin IIA mediated force-generating machinery, cells move with minimal traction forces at the cell periphery, thus demonstrating the remarkable ability of cells to adapt and migrate.

Original languageEnglish (US)
Pages (from-to)368-372
Number of pages5
JournalBiology Open
Volume2
Issue number4
DOIs
StatePublished - Apr 15 2013

Fingerprint

traction (mechanics)
Nonmuscle Myosin Type IIA
Traction
Nonmuscle Myosin Type IIB
myosin
Myosin Type II
cells
Small Interfering RNA
Machinery
Actins
Protein Isoforms
Adhesion
Cells
cell movement
Cell Movement
Focal Adhesions
Repair
actin
adhesion
Tissue

Keywords

  • Cell migration
  • Focal adhesion
  • Force sensor
  • Myosin II
  • Traction force

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Myosin IIA deficient cells migrate efficiently despite reduced traction forces at cell periphery. / Jorrisch, Melissa H.; Shih, Wenting; Yamada, Soichiro.

In: Biology Open, Vol. 2, No. 4, 15.04.2013, p. 368-372.

Research output: Contribution to journalArticle

Jorrisch, Melissa H. ; Shih, Wenting ; Yamada, Soichiro. / Myosin IIA deficient cells migrate efficiently despite reduced traction forces at cell periphery. In: Biology Open. 2013 ; Vol. 2, No. 4. pp. 368-372.
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